02404nam a2200253 a 4500 vtls000058774 KUKTEM 20251114204517.0 120327t2010 my a f m 000 0 eng d THE0004533(Local) 201905131550 azhar 201203270925 Fida 201203270925 Fida UMP TP248.65.P76 M39 2010 rs Bc. Mohd Mazlan Mohamed Isolation Of ß-Lactoglobulin and a-Lactalbumin from whey / Mohd Mazlan Mohamed Kuantan, Pahang : UMP, 2010 xii, 39 p. : ill. (some col.) ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Bibliography : p. [30]-32 Whey is a byproduct in cheese production. Whey solution is rich with proteins; the main proteins in whey are β-Lactoglobulin (β-Lg) (50%), α-Lactalbumin (α-La) (20%), bovine serum albumin (BSA) (10%) and immunoglobulin’s (Igs) (10%). Single protein has higher value then whey solution and has specific application. Hence this paper aims to isolate two of main whey proteins that are β-Lg and α-La due to their functions and higher value of pure protein. In this work, whey was prepared during casein coagulation from skim milk by acidic precipitation at pH below than 5 with 0.5 M hydrochloric acid. The experiments were conducted by AKTA Fast Protein Liquid Chromatography (FPLC), using size exclusion (packed by superdex TM 75 gel filtration media in an empty size exclusion XK column (60 ml)) and cation exchange column (HiTrap SP HP, 1ml column) in order to isolate both proteins from whey and 10% separating gel of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) was used to identify each protein form isolation steps and to determine the degree of purity of β-Lg and α-La. Silver staining was used in developing protein band on SDS gel. The experimental results concluded that both β-Lg and α-La can be isolated from other proteins in whey by using size exclusion and cation exchange fast protein liquid chromatography. Both β-Lg and α-La were visualized at 18 kDa and 14 kDa respectively, in SDS-PAGE. Proteins Separation Separation (Technology) 0 0 0 1 10000 10000 2019-09-04 0 TP248.65.P76 M39 2010 rs Bc. 0000063234 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5932 | TP248.65.P76 M39 2010 rs Bc. 0000063235 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3277 3283 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992