02149nam a2200241 a 4500 vtls000058590 KUKTEM 20251114204518.0 120306t2010 my a f m 000 0 eng d THE0004761(Local) 201905151057 smfh 201203061220 Fida 201203061219 Fida UMP TP414 .S65 2010 rs Bc. Siti Solehah Ahmad Effect of temperature and pH on glucose production using enzymatic hydrolysis / Siti Solehah Ahmad Kuantan : UMP, 2010 xv, 89 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Bibliography: p. 66-68 Enzymatic hydrolysis is one of the effective methods in glucose production due to enzyme properties which are highly specific and very sensitive. Therefore, this paper was designed to study the effect of temperature and pH on glucose production using enzymatic hydrolysis. By using enzymatic hydrolysis, cellulase from Trichoderma reesei was used and supplemented with cellobiase from Aspergillus niger for increasing the glucose production. Cellulose as a substrate was hydrolyzed by these enzymes to produce glucose and the amount of glucose production was determined by high-performance liquid chromatography (HPLC). The result showed that the highest glucose concentration was produced at the temperature of 50°C and pH 5.0 while the lowest glucose production was produced at 30°C and pH 6.5 which were 21.09 mg/ml and 3.55 mg/ml, respectively. By changes the temperature and pH above or below 50°C and pH 5.0 results in low glucose production because the enzyme was lose their three-dimensional functional shape due to disruption from unstable condition of the environment system. As a conclusion, all the requirement for complementary in the configuration of cellulose and enzyme must be considered seriously for the highest glucose production. Glucose 0 0 0 1 10000 10000 2019-09-04 0 TP414 .S65 2010 rs Bc. 0000063200 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5873 | TP414 .S65 2010 rs Bc. 0000063201 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3300 3306 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992