02123nam a2200253 a 4500 vtls000068066 KUKTEM 20251114204522.0 130110t2012 my a f m 000 0 eng|d THE0003878(Local) 201905161515 shah 201301101045 Fida UMP TP156.C5 A37 2012 rs Bc. Nur Aishah Amalin Ramjis Preparation of cation-exchange membrane chromatography through modification of polyamide membrane by grafting of acrylic acid monomer / Nur Aishah Amalin Ramjis Kuantan, Pahang : UMP, 2012 xiv, 59 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2012 Bibliography : p. 35-37 Packed bed chromatography is widely used for protein separation and purification. However some limitation of packed bed chromatography has been identified such as high-pressure drop, internal diffusion limitation and compaction for the soft beads. This encourages people to use membrane chromatography as alternative to the packed bed chromatography. In this study, membrane chromatography was developed from polyamide-based membrane by grafting acrylic acid (AA) monomer to produce cation-exchanger membrane chromatography. During grafting process, the concentration of AA monomer from 0.1 M to 1.0 M and grafting time from 15 min to 120 min was studied. The optimum monomer concentration was achieved at 0.5 M with a binding capacity of 443.39 mg BSA/g membrane. The optimum reaction time is 45 minutes which gives a binding capacity of 533.40 BSA mg/g membranes. The BSA adsorption capacity can be related to the amount of carboxyl group present in the modified membrane surface after grafting process. The successful of membrane chromatography preparation depend on the careful optimization the chemical reaction parameters involve. Chromatography Chromatography Analysis 0 0 0 1 10000 10000 2019-09-04 0 TP156.C5 A37 2012 rs Bc. 0000068487 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 6460 | TP156.C5 A37 2012 rs Bc. 0000068488 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3417 3423 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992