02335nam a2200253 a 4500 vtls000068064 KUKTEM 20251114204523.0 130109t2012 my a f m 000 0 eng|d THE0003879(Local) 201905161515 shah 201302041534 huda 201302041532 huda 201301091648 Fida 201301091558 Fida UMP TP156.C5 A45 2012 rs Bc. Nurul Alia Khalil Preparation of cation-exchange membrane chromatography by modification of polyamide membrane through grafting of methacrylic acid monomer / Nurul Alia Khalil Kuantan, Pahang : UMP, 2012 xiii, 43 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2012 Bibliography : p. 33-35 Nowadays, with the increase demand of protein production and purity, the purification cost of the protein also increases about 50 to 90%. Packed bed chromatography is widely used in protein separation. However, there are some limitations of the packed bed chromatography such as high-pressure drop, channeling problem and complicated scale up process. Most of these limitations can be overcome by membrane chromatography. The main objective of this research is to prepare cation-exchange membrane chromatography from polyamide membrane by chemical grafting of methacrylic acid monomer. Potassium persulfate and potassium metabisulfite was used to generate the radicals in the polyamide membrane and grafted with methacrylic acid monomer together with ethylene glycol dimethacrylate as a cross-linker. Different monomer concentration between 0.1 and 1.0M and monomer grafting duration from 15 – 120 min was studied toward producing high protein binding capacity membrane. Optimum time for grafting methacrylic acid onto polyamide membrane was 45 minutes with the binding capacity 599.6390 mg/g BSA at the monomer concentration of 0.1 M. Identification and optimization of critical parameter in grafting process is crucial for the development of high performance membrane chromatography materials. Chromatography Chromatography Analysis 0 0 0 1 10000 10000 2019-09-04 0 TP156.C5 A45 2012 rs Bc. 0000068489 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 6461 | TP156.C5 A45 2012 rs Bc. 0000068490 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 3450 3456 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992