Rajan Sinnathamby creator text theses my Kuantan, Pahang UMP 2012 monographic eng

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xiii, 48 p. : ill. (some col.) ; 30 cm. + 1 CD-ROM This study uses P. Chrysosporium and OPEFB hydrolysates (containing ferulic acid) for the fermentation process to convert ferulic acid in the oil palm empty fruit bunch (OPEFB) hydrolysates to vanillic acid and then to vanillin. The protein from the fermentation process is recovered using the cross flow filtration process. According to Converti et al. (2000), cross flow filtration is a key separation process due to their excellent fractionation of permeate and retentate. Cross flow filtration also economical. The scope of the research is to refine vanillin from fermentation broth using cross flow filtration and at the same time investigating the effect of membrane molecular weight cut off (MWCO), pump speed, and transmembrane pressure to filtration rate of vanillin via permeate flux. The immediate objective of this research project is to To refine vanillin from fermentation broth using cross flow filtration as the first step by comparing the performance of different molecular weight cut offs of membrane via permeate flux. Basic basal medium is prepared using Maltose 20g, Potassium dihydrogen phosphate 0.2g, Magnesium sulphate 0.5g, Ammonium tartrate 1.8g, Yeast extract 0.5g, Calcium chloride 1.3g Fermentation is done using P. chrysosporium with oil palm empty fruit bunch as the substrate (Barbosa et al., 2008). The mixture undergo centrifugation to remove insolubles after fermentation. Later the product produced is determined using ultra performance liquid chromatography and undergo centrifugation to remove insolubles. Supernatant from the centrifugation is subjected to cross flow filtration to concentrate the targeted protein. The protein recovered is tested using Folin Ciocalteau solution (reagent solutions) and then the absorbance is measured at 660 nm. Protein concentration of the sample is determined using the standard calibration curve (absorbance against protein concentration). The results is then analyzed using design expert software. From the design expert analysis could be concluded that the protein separation using cross flow filtration is very effective at optimum level of the factors involved. Based on the ANOVA analysis, the R-square value of 0.9863 and 0.9860 is achieved for permeate and retentate respectively showing that the experiment is significant and the mode equation for both permeate and retentate is acceptable. The factors involved shared contribution factors to the efficiency of the protein recovery for both permeate and retentate with molecular weight cut off being the dominant in both which contribute 72.4 % and 97.78 % respectively. While transmembrane pressure and pump speed contributed 14.6 % and 8.72 % for permeate and 0.69 % and 0.13 % for retentate. The optimal condition for all the factors are determined using the numerical method by analyzing the ramps. The optimal condition for molecular weight cut off, transmembrane pressure and pump speed is 30 kda to 40 kda, 0.5 bar to 0.6 bar and 250 rpm to 300 rpm respectively. Hence from the results obtained the objective is achieved. specialized Rajan Sinnathamby Project paper (Bachelor of Chemical Engineering in Biotechnology) -- Universiti Malaysia Pahang – 2012 Bibliography : 43-45 Vanillin THE0004326(Local) UMP 130716 20251114204541.0 vtls000073501