02967nam a2200265 a 4500 vtls000075742 KUKTEM 20251114204543.0 131028t2013 my da f abm 000 0 eng d THE0004001(Local) 201905151035 fauzi 201310280837 nabilah 201310280834 nabilah UMP TP156.E8 F37 2013 rs Bc. Nurfariha Fathmi Zakaria Application of ultrasound technique for Steviol Glycosides separation from mixtures of S.Rebaudiana stems and leaves using methanol / by Nurfariha Fathmi Zakaria Kuantan, Pahang : UMP, 2013 xii, 38 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 31-34 The mixture of s.rebaudiana stems and leaves will be extracted to get the stevioside. Stevioside is a diterpene steviol Glycoside which is produced by conventional process. It is used to sweeten soft drink,soya sauce, yoghurt and other food. It also good for diabetic patients. This research objective is to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves using ultrasound extraction technique and find their applications. The process normally include the extraction, pre-treatment, separation, purification and refining. These processes will use ultrasound technique and followed by High performance liquid chromatography analysis (HPLC). Ultrasound extraction technique is one of the techniques to extract the mixture of s.rebaudiana stems and leaves before the sample of the the mixture of s.rebaudiana stems and leaves is analyzed by HPLC. The mixture of s.rebaudiana stems and leaves will be in dry and powder condition before it extracted by ultrasound extraction technique using methanol as a solvent. Power level that used in the extraction is 280 W and the time range is between 10min to 50min at temperature range of 200C to 600C. After that the analysis was performed using High performance liquid chromatography (HPLC). Using 30 min, 40 0C and 80% methanol is the most suitable time, temperature and percent of solvent used to break the analyte and matrix bond and produce the steviol Glycoside in optimum yields. From this research we know that ultrasound extraction technique is most common technique and most suitable in order to extract the steviol glycosides from the mixture of s.rebaudiana stems and leaves. The optimum yield can afforded at extraction time of 30 minutes, extraction temperature of 40 0C and the percentage of methanol of 80%. Extraction kinetic of steviol glycosides approved the second-order kinetic yielding good R2 values of 0.997 and k values of 0.0301. Extraction (Chemistry) Stevia Sweeteners 0 0 0 1 10000 10000 2019-09-04 0 TP156.E8 F37 2013 rs Bc. 0000074605 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7002 | TP156.E8 F37 2013 rs Bc. 0000074606 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4016 4022 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992