03000nam a2200241 a 4500 vtls000075817 KUKTEM 20251114204546.0 131031t2012 my da f m 000 0 eng d THE0002270(Local) 201905311507 nazirah 201311281654 nabilah 201310311611 nabilah UMP QP752.O44 F37 2012 rs Bc. Nor Farahiyah Ghazali Production of omega-3 fatty acid from Nannochloropsis Sp. on the effects of carbon dioxide and light intensity / Nor Farahiyah Ghazali Kuantan, Pahang : UMP, 2012 xiii, 47 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012 Bibliography : p. 39-41 Omega-3 polyunsaturated fatty acid (PUFA) plays a vital role in a number of human health aspects substantially for the regulation of biological function, prevention and treatment of human diseases. Subsequently, humans cannot synthesize the omega-3 PUFA, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), thus, intake comes predominantly from dietary. Therefore, the microalgae was discovered as an alternative source of fatty acids compared to fish oil since it offers better purification essential and has no risk of chemical contamination. Moreover, it also acts as a carbon dioxide fixer by photosynthesis process in reducing the atmospheric carbon dioxide. There are major factors give affect for microalgae growth which is carbon source, irradiance level, temperature, nutrient availability and salinity. This study is carried out to investigate carbon dioxide effect in the specific range of 2%, 5%, 10% and 15% at flow rate of 0.2 L/min. Meanwhile, the irradiance levels are examined by using 31 µmol photons m-2 s-1, 82 µmol photons m-2 s-1 , 125 µmol photons m-2 s-1 and 156 µmol photons m-2 s-1 by applying one factor at time (OFAT) in maximizing the production of omega-3 fatty acids. The microalgae of Nannochloropsis sp. are cultured in f/2 medium with 300 ml working volume for 7 days of cultivation in an experimental system of photobioreactor. The omega-3 fatty acid compositions of Nannochloropsis sp. under different culture conditions are analyzed by lipid extraction and gas chromatography analysis in order to acquire the maximum amount of yield production. The results showed that the highest eicosapentaenoic acid productivity by Nannochloropsis sp. had been obtained at 2% of carbon dioxide aeration and 156 µmol photons m-2 s-1 which are 31.3121 mg/ml and 35.1339 mg/ml, respectively. The fatty acid accumulation of Nannochloropsis sp. could be prevailed to produce a high yield under their optimal conditions which are in maximal efficiency of carbon dioxide and high of light intensity. Omega-3 fatty acids 0 0 0 1 10000 10000 2019-09-04 0 QP752.O44 F37 2012 rs Bc. 0000074873 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7136 | QP752.O44 F37 2012 rs Bc. 0000074874 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4091 4097 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992