02357nam a2200241 a 4500 vtls000075132 KUKTEM 20251114204549.0 131007t2013 my da f m 000 0 eng d THE0002109(Local) 201905131640 yusri 201311141204 nabilah 201310231023 nabilah 201310071644 nabilah UMP QD272.C4 N35 2013 rs Bc. Wan Najibah Mohamad Preparation of ion exchange membrane chromatography by modification of Polyethersulfone membrane through UV grafting of [2-(Acryloyloxy) Ethyl] Trimethyl Ammonium Chloride and Acrylic Acid Monomer / Wan Najibah Mohamad Kuantan, Pahang : UMP, 2013 xiii, 56 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 38-40 Chromatographic separation of protein mixtures has become one of the most effective widely used means of techniques to purifying individual proteins. Packed bed chromatography is the common technique that is used configuration for the protein separation. However, packed bed chromatography has its some limitations during separation process such as high pressure drop and time consuming. Membrane chromatography then introduced to overcome the limitations of the packed bed chromatography. In the current research, polyethersulfone (PES) commercial membrane was converted into ion exchange (IEX) membrane chromatography by attaching [2-(acryloyloxy)ethyl] trimethyl ammonium chloride (AETMA) and acrylic acid (AA) monomer using UV light irradiation technique. The effect of AETMA and AA monomer concentration from 1.5 M to 2.0 M was studied. The IEX membrane was characterized in term of degree of grafting, changed of functional group as well as protein binding capacity using pure bovine serum albumin. For AETMA-grafted membrane, the binding capacity increase about the 65.32 % as the monomer concentration increase from 1.5 M to 2.0 M. While, for AA-grafted membrane, the binding capacity shows a huge increment when the monomer concentration was increase from 1.5 M to 2.0 M with amount 78.65 %. Chromatographic analysis 0 0 0 1 10000 10000 2019-09-04 0 QD272.C4 N35 2013 rs Bc. 0000074891 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7145 | QD272.C4 N35 2013 rs Bc. 0000074892 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4190 4196 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992