02402nam a2200241 a 4500 vtls000075157 KUKTEM 20251114204550.0 131008t2012 my da f m 000 0 eng d THE0002205(Local) 201905131718 yusri 201311181656 nabilah 201310221704 nabilah 201310081219 nabilah UMP QK495.C74 H39 2012 rs Bc. Nor Hayati Shahidan Extraction of stevioside from Stevia Rebaudiana leaves using cellulase / Nor Hayati Shahidan Kuantan, Pahang : UMP, 2012 xiv, 49 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2012 Bibliography : p. 38-42 Stevioside is a diterpene glycoside present in Stevia Rebaudiana leaves that has the ability to sweeten at rated between 70 to 350 times than sucrose (0.4% w/v). It has no calorific value. Unlike many low calorie sweeteners, stevioside is stable at high temperature. The objective of this research is to extract stevioside from stevia rebaudiana leaves by using cellulase from Aspergillus Niger. Acetate buffer and ethanol were used as a medium for enzyme and as a solvent, respectively. In this present study, the enzymatic extraction of stevioside from stevia rebaudiana leaves was carried out using cellulase with various parameters that affect the production of stevioside such as concentration of enzyme, incubation time and temperature. Cellulase was observed to give the highest stevioside yield (16230 ± 0.3 μg/ml) at 40oC. This indicated that the maximum temperature for cellulase activity was 40oC. The results signify that the enzymatic extraction method is an alternative to solvent based stevioside extraction, based on its higher efficiency. Thus, it can be concluded that the extraction of stevioside from Stevia rebaudiana leaves using cellulase can be maximized under the maximum conditions for the cellulase activity where the used of solvent can be minimized in degrading the cell wall Together with the maximum heat and correct combination of the solvent used, a new and efficient way of extracting high yield of stevioside can be obtained. Stevia rebaudiana 0 0 0 1 10000 10000 2019-09-04 0 QK495.C74 H39 2012 rs Bc. 0000074889 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7144 | QK495.C74 H39 2012 rs Bc. 0000074890 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4215 4221 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992