02305nam a2200253 a 4500 vtls000076092 KUKTEM 20251114204550.0 131113t2013 my da f m 000 0 eng d THE0002097(Local) 201905131634 yusri 201311131511 nabilah UMP QD79.C453 C43 2013 rs Bc. Chan, Fong Shiew Development of multiple interactions mixed matrix membrane chromatography using lewatit MP500 anion resin and lewatit CNP 105 cation resin for whey protein fractionation / Chan Fong Shiew Kuantan, Pahang : UMP, 2013 xv, 51 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 45-50 The conventional method to purify protein is by using packed bed column chromatography. However, this method had several limitations such as high pressure drop and limited flow rate operation. Membrane chromatography can be used to overcome the limitation of packed column but the preparation of adsorptive membrane requires harsh chemical modifications. Mixed matrix membrane (MMM) preparation concept can be used as an alternative route to prepare membrane chromatography by physical blending of adsorptive resin with membrane polymer solution. In the current research, multiple interactions MMM chromatography was developed for whey protein fractionation using 7.5 wt% CNP105 cation resin and 42.5 wt% MP500 anion resin relative to base polymer content. The resins were blend at different composition in EVAL and cellulose base polymer matrix. Based on HPLC and SDS-PAGE analysis, both acidic and basic whey proteins were bound to the multiple interactions MMM in single run of whey batch fractionation. The binding capacity for major acidic whey proteins using EVAL based MMM are 4.255 mg BSA bound/ g MMM, 60.887 mg α-Lac bound/ g MMM and 231.788 mg β-Lac/ g MMM. For CA based MMM, the binding capacity are 2.970 mg BSA bound/ g MMM, 42.392 mg α-Lac bound/ g MMM and 179.817 mg β-Lac/ g MMM. Ion exchange chromatography Whey 0 0 0 1 10000 10000 2019-09-04 0 QD79.C453 C43 2013 rs Bc. 0000074723 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7061 | QD79.C453 C43 2013 rs Bc. 0000074724 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4223 4229 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992