02372nam a2200265 a 4500 vtls000076095 KUKTEM 20251114204551.0 131113t2013 my da f m 000 0 eng d THE0004207(Local) 201905152027 farhana 201311131613 nabilah UMP TP159.A5 F38 2013 rs Bc. Nik Fatima Nek Kamarzaman Ultrasonic extraction of Clinacanthus nutans and its antioxidant activity / Nik Fatima Nek Kamarzaman Kuantan, Pahang : UMP, 2013 xii, 52 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 34-37 Clinacanthus nutans is one of herb plants that contain antioxidant. This natural antioxidant can be used commercially in food as well as pharmaceutical industries. This research explored antioxidant activity of Clinacanthus nutans (CN) and it was extracted by using ultrasonic-assisted extraction. Antioxidant activity of CN extract was determined by using free radical scavenging activity test and ferric reducing antioxidant power (FRAP) assay. Extraction process was carried out in ultrasonic bath at 28 kHz by using different solvent to solid ratio (ranging from 5mL/g to 50mL/g), temperature (ranging from 10 to 60 ºC), and sonication time (ranging from 0 min to 160 min). From result, it showed that the optimum extract of CN was obtained at 60 ⁰C, for extraction duration of 100 minutes with 10 mL/g liquor to solid ratio. The CN extract possessed antioxidant activity by scavenging DPPH with maximum effect of 61.08 %. The scavenging ability of CN extract has potency approximately 0.07 times of ascorbic acid. The CN extract also possessed reducing power to reduce ferric to its ferrous. 100 µg/mL of CN extract had same value of absorbance as 1.74µg/mL of ascorbic acid. This result proved that ferric reducing activity of CN extract was 57 times less powerful than ascorbic acid. In conclusion, antioxidant was extracted from Clinacanthus nutans by using ultrasonic extraction. Justicia Extraction (Chemistry) Antioxidants 0 0 0 1 10000 10000 2019-09-04 0 TP159.A5 F38 2013 rs Bc. 0000074729 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7064 | TP159.A5 F38 2013 rs Bc. 0000074730 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4243 4249 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992