02264nam a2200253 a 4500 vtls000076030 KUKTEM 20251114204553.0 131112t2013 my da f m 000 0 eng d THE0002227(Local) 201905241039 shamsul 201311121626 nabilah UMP QK896 .N37 2013 rs Bc. Nasihah Mohd Nor Enzymatic extraction of stevioside from Stevia Rebaudiana leaves using cellulase / Nasihah Mohd Nor Kuantan, Pahang : UMP, 2013 xv, 40 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 34-35 Stevioside is one of the components known as diterpene glycoside existing in Stevia Rebaudiana leaves which have 250 to 300 sweeter than sucrose at the concentration of 0.4% (w/v). It is used as sweetening agents and taste modifier mostly in food industry. Moreover, stevioside has no calorific value and suitable used in therapeutic especially in treating diabetic people. The objective of this research is to extract the stevioside from Stevia rebaudiana leaves by using cellulase from Aspergillus Niger.The medium for enzyme used is acetate buffer while ethanol is applied as solvent in the enzymatic extraction of stevioside by performing various parameters such as concentration of enzyme, incubation time and temperature. Enzymatic extraction method gives the highest concentration of stevioside (900μg/ml) at 50°C as maximum temperature. The concentration of cellulase at 2% (w/v) gives the highest concentration of stevioside (830μg/ml) and the incubation times of 60 minutes gives the maximum time required to complete the extraction process of stevioside.Therefore, it can be concluded that the extraction of stevioside from Stevia rebaudiana leaves using cellulase is a new efficient way of obtaining high concentration of stevioside and also can minimize the use of solvent and energy consumption in degrading the cell wall. Stevia rebaudiana Enzymes 0 0 0 1 10000 10000 2019-09-04 0 QK896 .N37 2013 rs Bc. 0000074637 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7018 | QK896 .N37 2013 rs Bc. 0000074638 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4287 4293 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992