02203nam a2200253 a 4500 vtls000076033 KUKTEM 20251114204553.0 131113t2013 my da f m 000 0 eng d THE0002259(Local) 201905241128 shamsul 201311130846 nabilah 201311130844 nabilah UMP QP609.P3 Y86 2013 rs Bc. Muhammad Yunus Zainal Abidin Stability of papain in aqueous organic solvent by reverse phase liquid chromatography / Muhammad Yunus Zainal Abidin Kuantan, Pahang : UMP, 2013 xiii, 49 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 37-38 Papain is one of the proteolytic enzymes found in the latex of the leaves and of the green fruit of the papaya tree (Carica papaya L.). It’s known to be highly used in the several industry like food, medical, brewing and many more. Hence, the objective of this research is to study the stability of papain in aqueous organic solvent and secondly to purify the papain using the AmberliteTM XAD7HP as the reverse phase liquid chromatography adsorbent. The research is based on experimental lab research. The effects of different concentrations of the water-miscible organic solvents acetonitrile and ethanol on the stability of papain in aqueous solution were studied. Batch adsorption using AmberliteTM XAD7HP was carried out to purify papain from papaya juice. The purified papain was analysed using the Bradford assay, the proteolytic papain activity assay and SDS PAGE. As for conclusion the of the study. The result obtained proved that papain has exhibited higher stability in aqueous acetonitrile than in ethanol under small margin. Decreases in the activity of the enzyme were observed at organic solvent concentrations above 60%. Papain with high purity was achieved by adsorption using the AmberliteTM XAD7HP. Papain Liquid chromatography 0 0 0 1 10000 10000 2019-09-04 0 QP609.P3 Y86 2013 rs Bc. 0000074735 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7067 | QP609.P3 Y86 2013 rs Bc. 0000074736 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4306 4312 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992