02404nam a2200253 a 4500 vtls000076039 KUKTEM 20251114204554.0 131113t2013 my da f m 000 0 eng d THE0004521(Local) 201905131527 azhar 201311130906 nabilah UMP TP248.65.P76 A35 2013 rs Bc. Ahmad Afifi Ibrahim Effect of pH and enzyme loading on the protein concentration during collocalia fuciphaga hydrolysis / Ahmad Afifi Ibrahim Kuantan, Pahang : UMP, 2013 xvi, 68 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 60-63 The purpose of this study is to investigate the effect of pH and enzyme loading on the concentration of protein during enzymatic hydrolysis of Collocalia Fuciphaga. C. Fuciphaga is refers to the bird nest from one swiftlet species that is used as the sample known to be highly nutritious. Bovine Serum Albumin (BSA), a standard protein solution was used as the benchmark for the reference of the protein concentration. A research had been proposed in this study to optimize the production of protein from C.Fuciphaga. The effect of changing of the parameters values during the enzymatic hydrolysis was studied and the higher protein concentrations produced were highlighted. The standard protein curve of Bovine Serum Albumin (BSA) is prepared first with several dilutions for protein standard curve. The results from two significant parameters in the experiment was taken and analyzed. The selection of parameters values was taken from the enzymes’ optimum condition where the pH value of Alcalase 2.4L is from pH 7 until pH 10 whiles the temperature is fixed at 50oC. Enzyme loading was varies from 1.5% to 3.0% (v/w). The sample was analyzed using UV-Vis Spectrophotometer to determine the concentration of protein in each sample. The optimum condition is found to be at extraction condition of pH 8.5 and enzyme concentration of 3.0% (v/w). Characterization of the sample had been done and validated the protein composition on the extracted sample. Proteins Hydrolysis 0 0 0 1 10000 10000 2019-09-04 0 TP248.65.P76 A35 2013 rs Bc. 0000074733 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7066 | TP248.65.P76 A35 2013 rs Bc. 0000074734 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4319 4325 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992