02227nam a2200265 a 4500 vtls000076071 KUKTEM 20251114204554.0 131113t2013 my da f m 000 0 eng d THE0004756(Local) 201905141152 smfh 201905141150 smfh 201311131041 nabilah UMP TP414 .F38 2013 rs Bc. Noor Fauzani Ayop Enzyme hydrolysis of old newspaper to produce simple sugar for fermentation process / Noor Fauzani Ayop Kuantan, Pahang : UMP, 2013 xv, 64 p. : ill. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 41-43 Production of fermentable sugar from lignocellulosic material was attempted by many researchers. Nowadays, this is because of plenty lignocellulosic biomass waste available around the world including Malaysia. Newspaper is a type of lignocellulosic sources because newspaper was made from softwood that contains cellulose, hemicelluloses and lignin. Cellulose component in newspaper is the major component with 45-50 %. The celluloses were able to be converted into simple sugar, which is glucose. Enzymatic hydrolysis is preferred for the conversion because less cost and high conversion. In this study, cellulase enzyme from Novozyme was used to hydrolyze newspaper for glucose production. By applying one-factor-at-time (OFAT), six parameters were being studied. They are surfactant ratio, enzyme loading, buffer pH, incubation temperature, agitation speed and incubation time. In all experiments, glucose production was monitored by DNS method. The result obtained show that at surfactant ratio 0.2:0.8 of TX-21: TWEEN-80, 1.0 ml of cellulase enzyme, at pH 6.0 when mixed with 0.2 g substrate at 50 oC and agitated at 200 rpm for 96 h is suitable for enzyme reaction. Those optimum parameters obtained gave the higher glucose production at 0.73 0.02 g/L. Glucose Hydrolysis Enzymes 0 0 0 1 10000 10000 2019-09-04 0 TP414 .F38 2013 rs Bc. 0000074763 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 7081 | TP414 .F38 2013 rs Bc. 0000074764 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 4330 4336 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*9992