03562nam a2200301 a 4500 vtls000075667 KUKTEM 20251114204555.0 131021t2013 my da f abm 000 0 eng d THE0003302(Local) 201905161520 irma 201710031602 aishah 201310211100 nabilah UMP TD812.5.M45 A95 2013 rs Thesis Abd. Aziz Mohd Azoddein Mercury removal from petroleum based industries wastewater by Pseudomonas putida ATCC 49128 in membrane bioreactor / Abd. Aziz Mohd Azoddein Kuantan, Pahang : UMP, 2013 xxiv, 267 p. : ill. ; 30 cm. + 1 CD-ROM Thesis (PhD in Chemical Engineering) -- Universiti Malaysia Pahang – 2013 Bibliography : p. 181-219 Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Microbes have been used to solve environmental wastewater problems for many years. The objective of the study is to remove mercury from petrochemical wastewater using Pseudomonas putida (P. putida), ATCC 49128 in membrane bioreactor to enhance water quality to meet EQA – Industries Effluent 2011 requirements. The approach of this study is focused on the removal or reduction of mercury concentration including development studies of pure culture of P. putida and growth related parameters involved in designing the membrane bioreactor system. Furthermore, this research also provide an understanding of P. putida behavior in rehydrating freeze-dried growing method, growth parameters and optimum operating condition in membrane bioreactor. A field survey on mercury concentrations at several petroleum based industrial plants in Peninsular Malaysia revealed a maximum reading of 2318 ppb which is higher than the Department of Environmental (DOE), Malaysia requirement for Standard B (50 ppb). Hence, treatment study of P. putida on mercury contaminated wastewater was conducted at mercury concentration in the range of 1000 – 4000 ppb. Optimum operating conditions of 24 hours acclimatization time, 180 rpm of orbital shaker speed, temperature of 37oC, pH 7 and nutrient concentration of 8 g/L were identified for P. putida growth in a shaked flask. The overall percentage of mercury removal for sample with 4000 ppb mercury in bioreactor is 99.60% for the first 6 hours and 99.80% removal after the microfiltration membrane system. Microfiltration membrane enhanced further the treatment of the wastewater by retaining the P.putida from escaping during the release of treated wastewater, reducing the turbidity by 94.2% (5.32 NTU) and concentration of suspended solids up to 60.4%. (0.09 mg/L). It was identified that the mechanism of mercury detoxification in the membrane bioreactor was based on reduction of Hg2+ to non-toxic Hg0 by mercury reductase enzyme produced by P. putida. The findings from this study can be used as references for future application of petroleum based industries wastewater treatment as well as other industries related to mercury contamination in their wastewater treatment plant such as gold mining, chemical industries, agriculture etc. Petroleum toxicity Water Pollutants, Chemical Water Purification Mercury wastes Pseudomonas http://ecollib.ump.edu.my/25593/ Access in library only 0 0 lcc 0 1 10000 10000 2019-09-04 0 TD812.5.M45 A95 2013 rs Thesis 0000077672 2019-09-04 00:00:00 1 2019-09-04 THESIS 0 0 lcc 0 1 10000 10000 2019-09-04 0 CD 7544 | TD812.5.M45 A95 2013 rs Thesis 0000077673 2019-09-04 00:00:00 1 2019-09-04 THESIS VIRTUA40 4354 4360 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*6502*6503*6504*8560*9992