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  <titleInfo>
    <title>Yeast protocols</title>
  </titleInfo>
  <name type="personal">
    <namePart>XIAO, WEI</namePart>
  </name>
  <typeOfResource>text</typeOfResource>
  <genre authority="marc">bibliography</genre>
  <originInfo>
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      <placeTerm type="code" authority="marccountry">nju</placeTerm>
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    <place>
      <placeTerm type="text">Totowa, N.J</placeTerm>
    </place>
    <publisher>Humana Press</publisher>
    <dateIssued>c2006</dateIssued>
    <dateIssued encoding="marc">2006</dateIssued>
    <edition>2nd ed.</edition>
    <issuance>monographic</issuance>
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  <language>
    <languageTerm authority="iso639-2b" type="code">eng</languageTerm>
  </language>
  <physicalDescription>
    <form authority="marcform">print</form>
    <extent>xi, 392 p. : ill. ; 23 cm.</extent>
  </physicalDescription>
  <tableOfContents>Basic investigations in saccharomyces cerevisiae / Brendan P.G. Curran and Virginia Bugeja -- Isolation of nucleic acids / Michelle Hanna and Wei Xiao -- Purification of yeast peroxisomes / Ben Distal and Astrid Kragt -- Isolation of yeast plasma membranes / Barry Panaretou and Peter Piper -- Isolation of yeast mitochondria / Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott -- Extraction of yeast lipids / Roger Schneiter and Günther Daum -- Two-dimensional gel electrophoresis of total yeast proteins / Hélian Boucherie and Christelle Monribot-Espagne -- Pulsed field gel electrophoresis of budding yeast chromosomes / Laura Maringele and David Lydall -- Analysis of yeast lipids / Roger Schneiter and Günther Daum -- Yeast fluorescence microscopy / Jirí Hasek --</tableOfContents>
  <tableOfContents>Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in schizosaccharomyces pombe / Michelle A. Alting-Mees ... [et al.] -- Yeast transformed by the LiAc/SS carrier DNA/PEG method / R. Daniel Gietz and Robin A. Woods -- Mutagenesis / Leslie Barbour, Michelle Hanna, and Wei Xiao -- Gene disruption in the budding yeast saccharomyces cerevisiae / Johannes H. Hegemann, Ulrich Güldener, and Gabriele J. Köhler -- Inducible degron and its application to creating conditional mutants / R. Jürgen Dohmen -- Synthetic lethal screen / Leslie Barbour and Wei Xiao -- Synthetic genetic array analysis in saccharomyces cerevisiae / Amy Hin Yan Tong and Charles Boone -- Two-dimensional agarose gel analysis of DNA replication intermediates / Alain T. Dandjinou ... [et al.] -- Chromatin assembly in a crude fraction from yeast cells / Karen M. Robinson and Michael C. Schultz -- Chromatin immunoprecipitation to study protein/DNA interactions in budding yeast / Elena Ezhkova and William P. Tansey --</tableOfContents>
  <tableOfContents>Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning / Zhengjian Zhang and Joseph C. Reese -- Study of transcriptional regulation using a reporter gene assay / Yu Fu and Wei Xiao -- Assessing telomeric phenotypes / Catherine LeBel ... [et al.] -- Controlled expression of recombinant genes and preparation of cell-free extracts in yeast / Zhigang Wang -- Production of heterologous proteins in yeast with the aid of the Hsp150-Carrier / Marja Makarow ... [et al.] -- Yeast two-hybrid system screening / R. Daniel Gietz -- Import of precursor proteins into isolated yeast mitochondria / Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling</tableOfContents>
  <targetAudience authority="marctarget">specialized</targetAudience>
  <note type="statement of responsibility">edited by Wei Xiao</note>
  <note>Includes bibliographical references and index</note>
  <subject authority="lcsh">
    <topic>Yeast fungi</topic>
    <topic>Biotechnology</topic>
    <topic>Laboratory manuals</topic>
  </subject>
  <subject authority="lcsh">
    <topic>Yeast fungi</topic>
    <topic>Laboratory manuals</topic>
  </subject>
  <subject authority="mesh">
    <topic>Yeasts</topic>
    <topic>physiology</topic>
    <topic>Laboratory Manuals</topic>
  </subject>
  <subject authority="mesh">
    <topic>Yeasts</topic>
    <topic>cytology</topic>
    <topic>Laboratory Manuals</topic>
  </subject>
  <subject authority="mesh">
    <topic>Yeasts</topic>
    <topic>isolation &amp; purification</topic>
    <topic>Laboratory Manuals</topic>
  </subject>
  <relatedItem type="series">
    <titleInfo>
      <title>Methods in molecular biology (Clifton, N.J.) ; 313</title>
    </titleInfo>
  </relatedItem>
  <identifier type="isbn">1588294374 (alk. paper)</identifier>
  <identifier type="isbn">9781588294371</identifier>
  <identifier type="uri">http://www.loc.gov/catdir/toc/ecip059/2005006244.html</identifier>
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    <url displayLabel="Table of contents">http://www.loc.gov/catdir/toc/ecip059/2005006244.html</url>
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    <recordCreationDate encoding="marc">090819</recordCreationDate>
    <recordChangeDate encoding="iso8601">20251117145535.0</recordChangeDate>
    <recordIdentifier source="KUKTEM">vtls000041768</recordIdentifier>
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