02698ntm a2200265 a 4500 vtls000084641 KUKTEM 20251117113230.0 141223t2014 my a f m 000 0 eng d THE0002051(Local) 201905131620 yusri 201712041259 huda 201412231522 fawwaz UMP QC609.C39 N37 2014 r Bc. Nasuha Ibrahim Effect of exposure time, bacteria concentration and culture age of escherichia coli and bacillus subtilis on the glass surface / Nasuha Ibrahim Kuantan, Pahang : UMP, 2014 x, 56 p. : ill. ; 30 cm. + 1 CD-ROM Faculty of Chemical & Natural Resources Engineering Project paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang – 2014 Bibliography : p. 50-56 This thesis presents the effect of the bacterial adhesion on the glass surface (hydrophilic surfaces) at different time exposure and bacterial concentration. The ability of Escherichia coli and Bacillus subtilis to attach to the surfaces depends mainly on the interaction of hydrophobic domains. However, E. coli and B. subtilis have evolved in different ways in order to manipulate the hydrophobic effect for their adherence on the solid surface. On the other hand, the surface properties e.g surface charges are inherently important and often regulate the mechanism of the bacteria adhesion. Besides that, adhesions of bacteria were also affected by culture media, exposure time of bacteria on glass surface, age and bacterial concentration. Both bacteria have different surface characteristic which also affect adhesion on the glass surface. Both bacteria were suspended in the phosphate buffer solution (pH 7.1) at different cell concentration (abs). The solution was suspended into glass container containing glass slide. The glass-bacterial solution was shake at 100 rpm and 30⁰C in the incubator shaker and sampling were done at 4 h, 8 h, 12 h and 24 h. From the researches that have been done B. subtilis easily adhere on the glass surface compared to E. coli, with 46.9% reduction in optical density reading observed at 600nm. Bacillus subtilis was exposed for 24 hour at cell concentration 0.8 abs. Meanwhile, E. coli result in less adhesion to the glass surface with only 29.8 % reduction in optical density. Yet, the time of exposure for E. coli was only 12 hour with cell concentration 1.0 abs Escherichia coli http://ecollib.ump.edu.my/18300/ Access in library only 0 0 0 1 10000 10000 2019-09-04 0 QC609.C39 N37 2014 r Bc. 0000091687 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 8610 | QC609.C39 N37 2014 r Bc. 0000091688 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 5112 5118 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6500*8560*9992