02094nam a2200253 a 4500 vtls000018577 KUKTEM 20251114204341.0 070308 000 0 eng d THE0004132(Local) 201905132118 farhana 201107131905 VLOAD 200908141305 VLOAD 200908141237 VLOAD 200703081235 farina UMP TP156.F4 N67 2006 rs Thesis Norhalmy B. Mohd Nor Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration / Norhalmy B. Mohd Nor Kuantan, Pahang : KUKTEM , 2006 xiii, 50 p. : ill. ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan dan Teknologi Malaysia - 2006 Bibliography: p. 44-46 ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB Fermentation Sugarcane 0 0 0 1 10000 10000 2019-09-04 0 TP156.F4 N67 2006 rs Thesis 0000021546 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 2039 | TP156.F4 N67 2006 rs Thesis 0000021547 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA 512 518 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*6501*9992