Norhalmy B. Mohd Nor creator text Kuantan, Pahang KUKTEM 2006 monographic eng

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xiii, 50 p. : ill. ; 30 cm. + 1 computer disc ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB Norhalmy B. Mohd Nor Project paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan dan Teknologi Malaysia - 2006 Bibliography: p. 44-46 Fermentation Sugarcane THE0004132(Local) UMP 070308 20251114204341.0 vtls000018577