03042ntm a2200229 a 4500001001400000003000700014005001700021008004100038020002200079040000800101100002600109245009000135260003400225300004800259500005600307502008800363504003100451520223800482650001802720650001702738856005702755vtls000085192KUKTEM20251117113241.0150203t2012    my a   f m    000 0 eng d  aTHE0004123(Local)  aUMP0 aNor Hidawati Hj Elias10aProduction of succinic acid from glycerol and glycerol waste /cNor Hidawati Hj Elias  aKuantan, Pahang :bUMP,c2012  axviii, 190 p. :bill. ;c30 cm. +e1 CD-ROM  aFaculty of Chemical & Natural Resources Engineering  aThesis (Master of Engineering in Bioprocess) -- Universiti Malaysia Pahang – 2012  aBibliography : p. 136-163	3 aSuccinic acid is extensively used globally for manufacture of synthetic resin, biodegradable  polymer, pharmaceutical product and food flavoring. Traditionally, succinic acid produced  through petrochemical route. The process is non-renewable and will affected pollution in  the environment. Therefore, more attention has been focused towards a friendly  environment processes for succinic acid production by way of fermentation process; which  is highly recommended nowadays. In this research, studies been done to produce succinic  acid through anaerobic fermentation of glycerol waste using Escherichia coli and to  investigate the effect of each factors that influence succinic acid production. The most  influential parameter for succinic acid production obtained via preliminary study of  parameter range selection were glycerol concentration, tryptone concentration, sodium  carbonate (Na2CO3) concentration , inoculums density, pH and incubation period. This  experiment has produced 0.973g/l succinic acid using One-factor-at-a-time (OFAT)  approach from commercial glycerol sample. These six variables screened to select the most  significant factor affected succinic acid production using fractional factorial design (FFD)  based on first order model term. Four factors selected while, two factors eliminated for the  next stage of experiment. Subsequently, full factor central composite designs (CCD) falling  under RSM employed to develop a mathematical correlation model between the significant  factors for physio-chemical variables of succinic acid production. The experiment has  produced 7.55g/l succinic acid from commercial glycerol sample. Besides, a second order  polynomial regression fitted and the model was adequate by R-squared 0.9864. Meanwhile,  under the optimum level, it is about 7.34g/l succinic acid being produced from commercial  glycerol and 7.32g/l of succinic acid produced from recovered glycerol. Overall, this  research shows glycerine pitch can be used safely and effective as a carbon source for  Escherichia coli to produce succinic acid. In addition, this research provides an exciting  opportunity to transform low-grade glycerine pitch into highly demanded chemical such  succinic acid 0aSuccinic acid 0aFermentation40uhttp://ecollib.ump.edu.my/3898/zLibrary access only