03308ntm a2200277 a 4500 vtls000085192 KUKTEM 20251117113241.0 150203t2012 my a f m 000 0 eng d THE0004123(Local) 201905132106 farhana 201710121529 aishah 201502031149 fawwaz UMP TP156.F4 H53 2012 r Thesis Nor Hidawati Hj Elias Production of succinic acid from glycerol and glycerol waste / Nor Hidawati Hj Elias Kuantan, Pahang : UMP, 2012 xviii, 190 p. : ill. ; 30 cm. + 1 CD-ROM Faculty of Chemical & Natural Resources Engineering Thesis (Master of Engineering in Bioprocess) -- Universiti Malaysia Pahang – 2012 Bibliography : p. 136-163 Succinic acid is extensively used globally for manufacture of synthetic resin, biodegradable polymer, pharmaceutical product and food flavoring. Traditionally, succinic acid produced through petrochemical route. The process is non-renewable and will affected pollution in the environment. Therefore, more attention has been focused towards a friendly environment processes for succinic acid production by way of fermentation process; which is highly recommended nowadays. In this research, studies been done to produce succinic acid through anaerobic fermentation of glycerol waste using Escherichia coli and to investigate the effect of each factors that influence succinic acid production. The most influential parameter for succinic acid production obtained via preliminary study of parameter range selection were glycerol concentration, tryptone concentration, sodium carbonate (Na2CO3) concentration , inoculums density, pH and incubation period. This experiment has produced 0.973g/l succinic acid using One-factor-at-a-time (OFAT) approach from commercial glycerol sample. These six variables screened to select the most significant factor affected succinic acid production using fractional factorial design (FFD) based on first order model term. Four factors selected while, two factors eliminated for the next stage of experiment. Subsequently, full factor central composite designs (CCD) falling under RSM employed to develop a mathematical correlation model between the significant factors for physio-chemical variables of succinic acid production. The experiment has produced 7.55g/l succinic acid from commercial glycerol sample. Besides, a second order polynomial regression fitted and the model was adequate by R-squared 0.9864. Meanwhile, under the optimum level, it is about 7.34g/l succinic acid being produced from commercial glycerol and 7.32g/l of succinic acid produced from recovered glycerol. Overall, this research shows glycerine pitch can be used safely and effective as a carbon source for Escherichia coli to produce succinic acid. In addition, this research provides an exciting opportunity to transform low-grade glycerine pitch into highly demanded chemical such succinic acid Succinic acid Fermentation http://ecollib.ump.edu.my/3898/ Library access only 0 0 lcc 0 1 10000 10000 2019-09-04 0 TP156.F4 H53 2012 r Thesis 0000091459 2019-09-04 00:00:00 1 2019-09-04 THESIS 0 0 lcc 0 1 10000 10000 2019-09-04 0 CD 8503 | TP156.F4 H53 2012 r Thesis 0000091460 2019-09-04 00:00:00 1 2019-09-04 THESIS VIRTUA40 5449 5455 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6500*6501*8560*9992