02153nam a2200253 a 4500001001400000003000700014005001700021008004100038020004300079040000800122100003000130245013700160260003500297300003500332504005000367505058300417520072801000650003301728650001501761650004901776650003501825650002001860650001901880vtls000049203KUKTEM20251117150251.0101213t2010    flua   f b    001 0 eng d  a9781420073492 (hardcover : alk. paper)  aUMP1 aKisaalita, William Ssempa10a3D cell-based biosensors in drug discovery programs :bmicrotissue engineering for high throughput screening /cWilliam S. Kisaalita  aBoca Raton :bCRC Press,c2010  axvii, 386 p. :bill. ;c24 cm.  aIncludes bibliographical references and index8 a(Publisher-supplied data) Biosensors and Bioassays -- Target-Driven Drug Discovery -- Comparative Genomics and Proteomics -- Comparative Structure and Function -- Chemical Microenvironmental Factors -- Spatial and Temporal Microenvironmental Factors -- Material Physical Property and Force Microenvironmental Factors -- Proteomics as a Promising Tool in the Search for 3D Biomarkers -- Readout Present and Near Future -- Ready-to-Use Commercial 3D Plates -- Challenges to Adopting 3D Cultures in HTS Programs -- Cases for 3D Cultures in Drug Discovery -- Ideal Case Study Design  a"This book is based upon cutting-edge research conducted in the authors lab (Cellular Bioengineering), which over the past decade has developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature, but there is a growing demand for live cell-based assays. Unlike biochemical ones, cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information"--Provided by publisher 0aPharmaceutical biotechnology 0aBiosensors 0aHigh throughput screening (Drug development)12aBiosensing Techniquesxmethods22aCells, Cultured22aDrug Discovery