03002ntm a2200277 a 4500 vtls000084596 KUKTEM 20251117113247.0 141222t2014 my a f m 000 0 eng d THE0004112(Local) 201905131408 farhana 201712051548 huda 201412221055 fawwaz UMP TP156.F4 A45 2014 r Bc. Nur Amira Maarof Production of poly (3-hydroxybutyrate) from pineapple waste / Nur Amira Maarof Kuantan, Pahang : UMP, 2014 xiv, 42 p. : ill. ; 30 cm. + 1 CD-ROM Faculty of Chemical & Natural Resources Engineering Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2014 Bibliography : p. 29-32 P(3HB) is the potential replacement of synthetic plastics due to its biodegradability and sustainability. Pineapple waste is one of the example of food waste that capable to produce P(3HB) as they contains sucrose, glucose, fructose and other nutrients that essential for production of bio plastics. Thus, the aim of this research is to employ the sugar present in pineapple waste as the carbon source for the bacteria producing biopolymer. The objectives are as follow; to determine the sugar content in the pineapple waste juice, to study the effect percent volume of juice from pineapple waste on production of P(3HB), to study the effect of pH on P(3HB) production. The method involve in this study are cultivation of bacteria, preparation of pineapple waste, preparation of inoculum, P(3HB) biosynthesis in shake flask, cell dry weight analysis and analysis of P(3HB) using HPLC. The results showed that glucose content in the mixture of skins and pulps is the highest with total of 28.80 g/L compared to fructose and sucrose. Fructose content come second with 21.25 g/L while sucrose has the lowest content in the PWJ with 20.28 g/L. The pineapple waste juice (PWJ) is then used as medium for the production of P(3HB). The fermentation medium with 50% and 100% volume of PWJ has the highest concentration of P(3HB) of 2.01 g/L (30th hour) and 1.49 g/L (44th hour) respectively. As the total dry mass production, the medium with 75% of PWJ has the highest concentration of P(3HB) with 14.42 g/L. The total concentration of P(3HB) for 50% and 100% PWJ are 10.52 g/L and 4.42 g/L respectively. The total average concentration of P(3HB) produced in the medium at pH 7 is 8.97 g/L. The highest concentration was found to be at the 44th hours with 2.15 g/L. As conclusion, the medium that consists of 75% PWJ and 25% ultra-pure water with the mixture of nutrient rich element at pH 7 is the most suitable medium to produce P(3HB) Biopolymers Fermentations http://ecollib.ump.edu.my/9807/ Access in library only 0 0 0 1 10000 10000 2019-09-04 0 TP156.F4 A45 2014 r Bc. 0000092110 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 8659 | TP156.F4 A45 2014 r Bc. 0000092111 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 5648 5654 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5000*5020*5040*5200*6500*6501*8560*9992