03669nam a2200313 a 4500001001400000003000700014005001700021008004100038020002800079020001800107020002400125039008400149040000800233090002200241245007600263260004100339300004600380490004000426504005000466505123500516505113501751650005302886700001702939830005902956856007803015952013303093999002703226999010203253vtls000041765KUKTEM20251117150440.0090819t2006    njua   f b    001 0 eng d  a1588293793 (alk. paper)  a9781588293794  a1597450057 (e-ISBN) 9a201204161235bidac201107132229dVLOADc201005141208dfauziy200908191730zshah  aUMP  aQH442.2 .N84 200600aNuclear reprogramming :bmethods and protocols /cedited by Steve Pells  aTotowa, N.J. :bHumana Press,cc2006  axiv, 333 p. :bill. (some col.) ;c24 cm.1 aMethods in molecular biology ;v325  aIncludes bibliographical references and index0 aNuclear transplantation in xenopus / John B. Gurdon -- Nuclear transfer in sheep / William A. Ritchie -- Protocols for nuclear transfer in mice / Shaorong Gao -- Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.] -- Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.] -- Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen -- Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada -- Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.] -- Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Håkelien, Kristine G. Gaustad, and Philippe Collas -- Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols -- Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Fátima Santos and Wendy Dean -- Observing s-phase dynamic of histone modifications with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert -- Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.] --0 aPluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.] -- Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.] -- Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fléchon -- Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young -- Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang -- Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung -- Histone modifications and transcription factor binding on chromatin: CHIP/PCR assays / Jaejoon Won and Tae Kook Kim -- In vivo genomic footprinting using LM-PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer -- Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer 0aCell nucleixTransplantationvLaboratory methods1 aPells, Steve 0aMethods in molecular biology (Clifton, N.J.) ;vv. 325413Table of contentsuhttp://www.loc.gov/catdir/toc/ecip0516/2005020464.html  00104070a10000b10000d2019-09-04g546.94l0oQH442.2 .N84 2006p0000046759r2019-09-04 00:00:00t1v546.94w2019-09-04yOPEN  aVIRTUA40c58571d58577  aVTLSSORT0080*0200*0201*0202*0400*0900*2450*2600*3000*4900*5040*5050*5051*6500*7000*8300*8560*9991