02267nam a2200241 a 4500 vtls000058721 KUKTEM 20251117113258.0 120319t2010 my da f m 000 0 eng d THE0004134(Local) 201905132119 farhana 201203191122 Fida 201203191120 Fida UMP TP156.F4 N677 2010 rs Bc. Norsalita Abd Talib Optimization of pullulanase activity through design of experiment (DOE) / Norsalita Abd Talib Kuantan, Pahang : UMP, 2010 xiii, 55 p. ; 30 cm. + 1 CD-ROM Project paper (Bachelor of Chemical Engineering (Biotechnology) -- Universiti Malaysia Pahang - 2010 Includes bibliographical references Enzyme has been used widely in industries today due to its capability in catalyzing various types of biochemical reactions. However, the high cost of enzyme gives problems to the enzyme industries. A research was done to optimize the pullulanase activity from Bacillus Acidopullulyticus using experimental design. Two level Full Factorial design was applied in the screening process of four parameters for pullulanase activity and only three parameters were recognized as significant and effective factors for pullulanase activity. The three significant parameters were pH, enzyme and substrate concentration. Thus the three parameters were selected for further optimization studies using response surface methodology (RSM) in Design Expert version 7.1.6 software with Central Composite Design (CCD) mode. Thirty four sets of experiments with different parameter values were suggested. The predicted optimum values with R2 of 0.8729 for pH, enzyme concentration, substrate concentration and pullulanase activity were 6.60, 11.11 U/ml, 4.44% (w/v) and 3.17 U/ml respectively. One validation experiment was run using the optimized parameter and resulted 3.05 U/ml pullulanase activities as compared to the suggested values of 3.17 U/ml. As a conclusion, this research was successful to increase the pullulanase activity with a minimum numbers of experiments. Pullulanase 0 0 0 1 10000 10000 2019-09-04 0 TP156.F4 N677 2010 rs Bc. 0000063267 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 5948 | TP156.F4 N677 2010 rs Bc. 0000063268 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 5993 5999 VTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992