03072nam a2200277 a 4500 vtls000053390 KUKTEM 20251117150522.0 110531t2011 nyua f 001 0 eng d 9781607619123 201110180944 asmadi 201107140051 VLOAD 201105311551 irma UMP QP550 .P76 2011 Protein chromatography : methods and protocols / edited by Dermot Walls and Sinéad T. Loughran New York, NY : Springer, 2011 x, 527 p. : ill. (some col.) ; 27 cm. Methods in molecular biology, 1940-6029 ; v. 681 Includes bibliographical references and index A digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases Proteomics Proteins Analysis Chromatographic analysis Walls, Dermot Loughran, Sinéad T. 0 0 0 0 10000 10000 2019-09-04 463.03 0 QP550 .P76 2011 0000060130 2019-09-04 00:00:00 1 463.03 2019-09-04 OPEN VIRTUA40 59971 59977 VTLSSORT0080*0200*0400*0900*2450*2600*3000*4900*5040*5050*6500*6501*6502*7000*7001*9991