04347ntm a2200373 i 4500 vtls000103188 KUKTEM 20251117113349.0 180329s2018 my da f am 000 0 eng d THE0001330(Local) 201905141043 asmadi 201803291154 saini UMP eng UMP rda FTeK .A95 2018 r Thesis Nik Nor Aziati Abd Aziz, author. Optimization of succinic acid production from immobilized escherichia coli / Nik Nor Aziati Abd Aziz Kuantan, Pahang : UMP, 2018 © 2018 xiii, 126 pages : illustrations (some color), chart ; 30 cm. + 1 CD-ROM text rdacontent text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Engineering Technology Thesis (Doctor of Philosophy in Bio-Process Engineering) -- Universiti Malaysia Pahang – 2018 Includes bibliographical references Succinic acid can be produced from glycerol residue by fermentation of immobilized Escherichia coli. Increament of the price of succinic acid is one of the factors to produce the succinic acid from the waste of glycerol. As a by product of oleochemical production, glycerol has now become an abundant and cheap source of carbon. To reduce the production cost, the glycerol waste is an alternative substrate for succinic acid production. Besides, by using immobilization method, the cells can be reused for succinic acid production.Therefore, this study was aimed to produced succinic acid using glycerol recovered from industrial waste oleochemicals, with E. coli that have been immobilized. Comparison between fermentation process using free and immobilized E-coli is carried out by considering the mass of the substrate, the density of inoculum and fermentation time. Succinic acid that has been produced from immobilized cell fermentation was optimized using response surface methodology. The study also identified the characteristics of immobilized cells including cell stability and the ability to recycle beads during the fermentation process. High performance liquid chromatography was used to analyze the concentration of glycerol and succinic acid. While Fourier transform infrared spectroscopy was used to analyze the functional groups of glycerol. One of the main studies was the kinetic study of the free and immobilized cells, which studied the effect of the substrate on the growth profile of the microorganism and thus, the production of succinic acid. The results obtained for the maximum specific growth rate μmax (hr-1) was 0.031 h-1 and the KS was 5.11 g for the immobilized cells, while for the free cells, the maximum growth rate was 0.029 h-1 and KS of 4.03 g. The difference between the immobilized and free cells was about 6.9 % for the μmax (hr-1) and 26.8 % for the KS. The results proved that the substrate concentration is one of the factors that may affect the microorganism growth rate. To optimize the parameters for scale-up purposes the optimum conditions observed for succinic acid fermentation process were time 3.31 h, mass substrate 40 g, and inoculum density 15%. At these optimum conditions, 124.09 g/L of succinic acid was obtained. The total yield obtained was 5.95 g of succinic acid. This study was successfully developed to produce and improve the succinic acid production from renewable glycerol residue using the immobilized cell method. For the immobilized cells, the selection method was important for high production of succinic acid. To reduce the cost of succinic acid production, the immobilized cells have the advantage of been able to be reused up to 6 cycles for succinic acid production. A further study should be carried out to investigate the structure of the beads for high production of succinic acid using the entrapment method. Faculty of Engineering Technology Dissertations Universities and colleges Disertations Theses 0 0 lcc 0 1 10000 10000 2019-09-04 0 FTeK .A95 2018 r Thesis 0000122616 2019-09-04 00:00:00 1 2019-09-04 THESIS 0 0 lcc 0 1 10000 10000 2019-09-04 0 CD 11245 | FTeK .A95 2018 r Thesis 0000122617 2019-09-04 00:00:00 1 2019-09-04 THESIS VIRTUA40 7407 7413 VTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3361*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992