03617ntm a2200325 i 4500001001400000003000700014005001700021008004100038020002200079040002300101100005500124245015100179264003400330264001200364300008000376336002100456336002100477337002500498337002300523338002300546338003000569347002400599500005100623502008400674504004000758520237200798610006603170650004403236650001103280vtls000103163KUKTEM20251117113357.0180326s2017    my da  f am   000 0 eng d  aTHE0005460(Local)  aUMPbengcUMPerda0 aTengku Nor Hidayati Tengku Zainal Abidin,eauthor.10aKaedah al-istibrā' haiwan akuatik menurut perspektif Islam dan sains akuakultur :bpangasius sutchii /cTengku Nor Hidayati Tengku Zainal Abidin 1aKuantan, Pahang :bUMP,c2017 4c© 2017  axvii, 111 pages :billustrations (some color), charts ;c30 cm. +e1 CD-ROM  atext2rdacontent  atext2rdacontent  aunmediated2rdamedia  acomputer2rdamedia  avolume2rdacarrier  acomputer disc2rdacarrier  atext filebPDF2rda  aCentre for Modern Languages and Human Sciences  aThesis (Master of Science in Halal Food) -- Universiti Malaysia Pahang – 2017  aIncludes bibliographical references3 aHalal is the Islamic term that refers to something lawful or permissible. As such, undoubtedly halal animal can be consumed by Muslim once slaughtered properly according to Islamic teaching. However, in the case of halal animals that fed with contaminated food/filth (najis) continuously, the status of the animal according to majority of the scholars, is changed to al-jallālah. Animals fed with najasa are categorized as al-jallālah (contaminated animals) are only allowed to be consumed if they are quarantined for a certain period of time. Duration of quarantine varies according to the type of animals, that between one to forty days. During this quarantine period the animals will undergo a natural purification process or istiḥālah. Patin fish (pangasius sutchii) that is commonly consumed in Malaysia could be fed on najasa.like pig organs. During the quarantine or al-istibrā', natural purification process/istiḥālah will take place in fish digestive system. This study was carried out to estimate the istiḥālah period for patin fish after feeding with pig offal, based on the absence of DNA porcine in patin fish stomach and to suggest the sufficient period/time of istiḥālah for patin fish. Quarantine period is determined when there is completely no traces of DNA porcine from the fish. Detection of DNA porcine was done by using PCR and RT-PCR. The findings show detection of DNA porcine in fish stomach that fed pig organs, while DNA porcine was not detected on the skin and fish fillet during zero (0) hour quarantine period. After the 20 hours quarantine, DNA porcine was not detected in the stomach of the fish. The volume of water used varies between (20%, 50%, and 80%) for purification of patin from pig contamination. DNA porcine was not detected in the stomach of fish which were quarantined for 24 hours using the volume of water 20%, 50%, and 80%. Thus, the al-istibra‟ method can solve the issue of al-jallālah animals and further enhance the Malaysia aquaculture industry. Extraction of genomic DNA from tissue of different fish part, water and pig offal with the application of RNase A, improved the quality of DNA obtainded for most of molecular application based on the DNA purity. The total DNA yield is different among the sample this might be due to the different structure and compactness of the fish tissue.20aCentre for Modern Languages and Human SciencesxDissertations 0aUniversities and collegesxDisertations 0aTheses