03607ntm a2200373 i 4500 vtls000102809 KUKTEM 20251117113359.0 180118s2017 my da f a m 000 0 eng d THE0000981(Local) 201905241626 nazirah 201802081544 fateeha 201802021152 fateeha 201801231143 fateeha 201801181553 fateeha UMP eng UMP rda FKKSA .N87 2017 r Bc. Nursyahira Mohd Rafi, author. Effect of freeze and thaw cycle and incubation period on the solubilisation of inclusion body protein / Nursyahira Mohd Rafi Kuantan, Pahang : UMP, 2017 © 2017 xiii, 70 pages : illustrations (some color), charts ; 30 cm. + 1 CD-ROM text rdacontent text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Chemical & Natural Resources Engineering Project Paper (Bachelors of Chemical Engineering) -- Universiti Malaysia Pahang – 2017 Includes bibliographical references Overexpression of recombinant protein in bacteria result in the formation of inactive protein. These inactive proteins associate forming insoluble protein aggregates which is referring to inclusion bodies (IBs). Generally, IBs are pure and the aggregated protein inside it has native-like secondary structure which is a bioactive protein. To recover the insoluble and active protein is a major problem encountered. Solubilisation does play a crucial role by unfolded the protein and thus help it to refold properly so that functional bioactive protein can be recovered. Example of mild solubilisation method using low concentration of urea and combine with freeze and thaw method has been proven to increase the efficiency of the recovering of bioactive protein. For freeze and thaw process there are factors that affect the overall process which are freezing incubation period and number of process cycle. Incubation period affect the process by determining the amount of stress needed to be applied so that unfolding process can occur. Number of cycle does affect the protein stability in terms of the occurrence of protein degradation probability. Thus the objective of this research is to investigate effect of freeze and thaw cycle an incubation period on the solubilisation of IBs. For incubation period the experiment was conducted between 1 to 4 days whereas for freeze and thaw cycle experiment conducted between cycle 1 to 4. Moreover, the performance of these two parameters were analysed using native-polyacrylamide gel electrophoresis (n-PAGE) to determine the functional for enhance green fluorescent protein (EGFP) amount and Bradford assay to determine the total protein amount. In this study, incubation period did affected the performance of solubilisation rate in which the IBs being solubilised and then proceed for refolding process and has been proven achieved active form of EGFP. For number of process cycle, it did not affect the solubilisation rate on determining the amount of functional EGFP recovered. Faculty of Chemical & Natural Resources Engineering Dissertations Universities and Colleges Dissertations Theses 0 0 0 1 10000 10000 2019-09-04 0 FKKSA .N87 2017 r Bc. 0000122138 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 CD 11149 | FKKSA .N87 2017 r Bc. 0000122139 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 7673 7679 VTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3361*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992