03029ntm a2200349 i 4500 vtls000103684 KUKTEM 20251117113402.0 180531s2018 my a f am 000 0 eng d THE0001430(Local) 201905211441 asmadi 201805311438 saini UMP eng UMP rda FTeK .T363 2018 r Bc. Tan, Darlene Tzet Lene, author. Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry / Darlene Tan Tzet Lene Kuantan, Pahang : UMP, 2018 xiii, 71 pages : illustrations (some color) ; 30 cm. + 1 CD-ROM text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Engineering Technology Project Paper (Bachelor Of Manufacturing Engineering Technology (Pharmaceutical) -- Universiti Malaysia Pahang – 2018 Includes bibliographical references The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at 𝑅𝑓 value of 0.22. However, in HPLC analysis, the caffeic acid was not detected in sample at the retention time of 3.82 minutes as per reference of the standard caffeic acid chromatogram. Split peak and baseline drift were also observed in WEP during HPLC analysis and requires troubleshooting process. WEP showed lower amount of total flavone, flavonols and total phenolic contents as compared to ethanolic extracted peopolis (EEP) and olive oil extracted propolis (OEP). For TLC, chrysin components was detected in EEP whereas none of the components were detected by OEP. For HPLC, caffeic acid were not detected all three propolis extracts. Faculty of Engineering Technology Dissertations Universities and colleges Disertations Theses 0 0 0 1 10000 10000 2019-09-04 0 CD 11344 | FTeK .T363 2018 r Bc. 0000122923 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 FTeK .T363 2018 r Bc. 0000122922 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 7764 7770 VTLSSORT0080*0200*0400*0900*1000*2450*2640*3000*3360*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992