03537ntm a2200373 i 4500 vtls000105308 KUKTEM 20251117113405.0 180926t20182018my a f a m 001 0 eng d THE0000861(Local) 201905271629 nazirah 201809261549 fateeha UMP eng UMP rda FKKSA .B39 2018 r Thesis Ahmad Bazli Bustary, author. Biological treatment of petrochemical effluent containing benzene, toluene and xylene (BTX) using pseudomonas putida ATCC49128 / Ahmad Bazli Bustary Kuantan, Pahang : UMP, 2018 © 2018 xiii, 129 pages : illustrations (some color) ; 30 cm. + 1 CD-ROM text rdacontent text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Chemical & Natural Resources Engineering Thesis (Master of Engineering (Chemical)) -- Universiti Malaysia Pahang – 2018 Includes bibliographical references Benzene, toluene, and xylene (BTX) are some examples of aromatic hydrocarbons that are found in petrochemical wastewater. Liquid and gaseous forms of the same are frequently present in municipal treatment plants. In this research, Pseudomonas putida ATCC 49128 was selected for use in the experiments owing to its ability to safely remove the aforementioned aromatic compounds without giving rise to the formation of other hazardous chemicals during the treatment of wastewater. The said experiments were aimed to study P. putida in terms of (1) its growth patterns under normal conditions and in concentrations of BTX, (2) its ability to remove different concentrations of BTX in a shake flask, as well as (3) its ability to remove BTX from actual petrochemical wastewater and simulated solutions. Repeated measurements of BTX concentrations at a petrochemical industrial wastewater effluent tank in Peninsular Malaysia revealed that the maximum values for benzene, toluene, and xylene were 55.2 ppm, 26.9 ppm, and 32.8 ppm respectively. Subsequently, the results of the treatment study showed that the optimal conditions for the growth of P. putida were an orbital shaker speed of 180 rpm, temperature of 37°C, pH 7, and P. putida-nutrient ratio of 1:9. Next, P. putida was made to remove different concentrations of simulated BTX for 48 hours; this procedure was repeated for actual petrochemical wastewater of pH 6, 7, and 8 respectively. This process was analysed using gas chromatography (GC-FID). Evidently, the bacteria were able to reduce the concentrations of BTX in actual petrochemical wastewater (of pH 7) by 85%, 77%, and 70% respectively; for the simulated BTX solution, the values were 87%, 80%, and 72% respectively. In the single simulated BTX solution, the reduction in the concentration of toluene (97%) exceeded those of benzene (93%) and xylene (62%). To conclude, the usage of P. putida ATCC49128 is an effective biological method to reduce the concentrations of BTX in petrochemical wastewater. Faculty of Chemical & Natural Resources Engineering Dissertations Universities and colleges Disertations Theses 0 0 lcc 0 1 10000 10000 2019-09-04 0 FKKSA .B39 2018 r Thesis 0000125012 2019-09-04 00:00:00 1 2019-09-04 THESIS 0 0 lcc 0 1 10000 10000 2019-09-04 0 CD 11594 | FKKSA .B39 2018 r Thesis 0000125013 2019-09-04 00:00:00 1 2019-09-04 THESIS VIRTUA40 7852 7858 VTLSSORT0080*0200*0400*0900*1000*2450*2640*2641*3000*3360*3361*3370*3371*3380*3381*3470*5000*5020*5040*5200*6100*6500*6501*9992