02086nam a2200241 a 4500 vtls000045111 KUKTEM 20251114204354.0 100208t2009 my a f 000 0 eng d THE0002279(Local) 201905241213 shamsul 201206191416 ida 201107132106 VLOAD 201002081624 kam UMP QR84.5 .I58 2009 rs Bc. Intan Noorleana Rosdi Growth optimization of N-fixer, isolated from biofertilizer / Intan Noorleana Bt Rosdi Growth optimization of n-fixer, isolated from [electronic resource] Kuantan : UMP, 2009 35 p. : ill. (some col.) ; 30 cm. + 1 computer disc Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009 The objectives of this experiment were to obtain the optimum temperature, pH and the requirement of carbon sources needed for the growth of N-Fixer bacteria. This research can be divided into two parts which are the standard curve preparation followed by optimization of the experiments. Three standard curves needs to be constructed; growth, glucose and CFU curves. These three curves will be used in optimizing the growth condition of N-fixer. Set of experimental works were designed by DOE software using full factorial function. Based on the experiment results, it shows that the optimum pH and temperature for the growth of N-Fixer is at pH 6 and 30°C respectively. As for the requirement of carbon sources, 10 g/L was applicable to avoid the surplus glucose become toxic to microorganisms. As the conclusion, the concentration of glucose plays a major role in the fermentation process (18 hours) either to increase the number of N-fixer cell or to decrease it. Therefore, by maintaining it at 10g/L the number of cell can achieve the upper limit to where it can be produced which is about 1.6834E8 cell/mL . Bacterial growth 0 0 0 1 10000 10000 2019-09-04 0 CD 3924 | QR84.5 .I58 2009 rs Bc. 0000042794 2019-09-04 00:00:00 1 2019-09-04 PSM 0 0 0 1 10000 10000 2019-09-04 0 QR84.5 .I58 2009 rs Bc. 0000042793 2019-09-04 00:00:00 1 2019-09-04 PSM VIRTUA40 892 898 VTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*9992