03277ntm a2200373 i 4500 0 0 lcc 0 6 REF 10000 10000 2020-10-15 0 FTKKP .D56 2019 r Thesis T000000962 2020-10-15 00:00:00 1 2020-10-15 THESIS 0 0 lcc 0 0 REF 10000 10000 2020-10-15 0 CD12708 T000000963 2021-12-06 00:00:00 1 2020-10-15 THESIS 94356 94362 MY-KuUP 20251125105736.0 t||||fr|||| 000 0 ta 201015t2 2 m a|||fram|| 000 0 eng d THE0008914(Local) hardback UMP eng UMP rda FTKKP .D56 2019 r Thesis Nur Dini Mat Jusoh, author. Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste / Nur Dini Mat Jusoh Kuantan, Pahang : UMP, 2019 © 2019 xi, 97 pages : illustrations (some color) ; 30 cm. + 1 CD-ROM text rdacontent text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Chemical and Process Engineering Technology Thesis (Master of Science) -- Universiti Malaysia Pahang – 2019 Includes bibliographical references Fructooligosaccharides (FOS), which are considered as inulin-type fructans, represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. Prebiotics are considered as additives of functional food due to the ability to promote host health by increasing the growth of probiotic microorganisms. In this study, FOS were produced by using enzymatic reaction of the phytoenzymes from Ananas Comosus waste.The characterisation of invertase and the phytoenzymes of A. Comosus waste was performed prior to FOS production by using Fourier transfrom infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), and ultraviolet-visible (UV-Vis) spectroscopy. FOS obtained from phytoenzymes and invertase showed similarities in terms of complex protein compounds. FTIR results showed that both invertase and the phytoenzymes from A.comosus waste give stretching from 1640.60 cm-1 and 1649.99cm-1 respectively and it is N-H bond. The chromatographic profile of the reaction product from the phytoenzyme of A.comosus waste revealed the presence of sucrose, fructose, glucose and FOS namely, kestose, and nystose. The production of FOS proceeds by fractional factorial design (FFD) central composite design (CCD) statistical methods with the optimisation parameters of reaction time, sucrose concentration, pH, phytoenzyme concentration and temperature using Design Expert version 7.0. All of these responses were screened by FFD method which showed that all the parameters were significant response to produce FOS. CCD method evaluates that pH (6) and temperature 60˚C was producing maximum FOS at 211.334(g/mL). The validation of empirical equations gained from the optimisation study was performed in laboratory scale with only 0.828 % error. As a conclusion, this study has successfully produced FOS using a new approach of alternative source which is from the phytoenzymes of A.Comosus waste. Faculty of Chemical and Process Engineering Technology Dissertations Universities and colleges Dissertations Theses lcc THESIS