03081ntm a2200373 i 4500 0 0 lcc 0 1 REF 10000 10000 2020-10-23 0 FSTI .M37 2019 r Thesis T000000950 2021-03-15 00:00:00 1 2020-10-23 THESIS 0 0 lcc 0 1 REF 10000 10000 2020-10-23 0 CD12702 T000000951 2021-12-06 00:00:00 1 2020-10-23 THESIS 94585 94591 MY-KuUP 20251125105750.0 t||||fr|||| 000 0 ta 201023t2 2 m a|||fram|| 000 0 eng d THE0008933(Local) hardback UMP eng UMP rda FSTI .M37 2019 r Thesis Marina Mohd Sham, author. Extraction and characterization of potential glycosaminoglycans (GAGS) from blue-spotted stingray for anticancer study / Marina Mohd Sham Kuantan, Pahang : UMP, 2019 © 2019 xiii, 89 pages : illustrations (some color) ; 30 cm. + 1 CD-ROM text rdacontent text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Industrial Sciences and Technology Thesis (Master of Science (Biotechnology)) -- Universiti Malaysia Pahang – 2019 Includes bibliographical references This study was carried out to find a new possible halal source of glycosaminoglycans (GAGs) for potential anticancer agent. The under-valued and rarely-bought cartilaginous Blue-spotted stingray was selected for the extraction and characterisation of GAGs. The objectives of the study are to isolate and extract the GAGs from the selected stingray and to characterise the GAGs by using characterization assay, FT-IR and NMR. Extraction was conducted to get the crude of sulfated GAGs from the Blue-spotted stingray. The crude GAGs obtained from the extraction was characterized by using Blyscan and Carbazole quantification assays. Blyscan assay result showed that the total sulfated GAGs amount obtained from the total crude GAGs is 3.9628 g. Quantification by carbazole assay strongly suggested that the crude extract contains 4.7480 g of uronic acids. The crude extract was then analysed by using Fourier Transform Infrared Resonance (FT-IR) and Nuclear Magnetic Resonance (NMR) to confirm its functional groups and molecular structures. From the FT-IR and NMR analysis, the spectra showed the presence of glycosaminoglycans in the crude extract of Blue-spotted stingray. The anticancer property of the crude extract was then studied by using human adenocarcinoma A549 (lung cancer). The results showed that crude GAGs can inhibit the A549 cells depends on the concentration used. As conclusion, blue-spotted stingray can be a halal source of potential GAGs as the yield of the crude GAGs extracted is comparable to the previous research findings. Furthermore, it showed cytotoxic property as it can inhibit the cancer cells growth. In future, the extracted GAGs from blue-spotted stingray could be a promising source of anticancer agent. Faculty of Industrial Sciences and Technology Dissertations Universities and colleges Dissertations Theses lcc RESTRICT