02629ntm a2200385 i 4500 0 0 lcc 0 1 REF 10000 10000 2020-11-24 0 FIST .S27 2012 r Bc. T000001317 2021-03-11 00:00:00 1 2020-11-24 THESIS 0 0 lcc 0 0 REF 10000 10000 2020-11-24 0 CD12816 T000001318 2021-12-06 00:00:00 1 2020-11-24 THESIS 95072 95078 MY-KuUP 20251125105826.0 t||||fr|||| 000 0 ta 201124t2 2 m a|||fram|| 000 0 eng d THE0009008(Local) hardback UMP eng UMP rda FIST .S27 2012 r Bc. Sasikala, Mahalingam, author. Cytotoxic activity of different fractions of tinospora crispa stems extracts / Sasikala A/P Mahalingam Kuantan, Pahang : UMP, 2012 © 2012 xvi, 66 pages : illustrations (some color) ; 30 cm. + 1 CD-ROM text rdacontent text rdacontent unmediated rdamedia computer rdamedia volume rdacarrier computer disc rdacarrier text file PDF rda Faculty of Industrial Sciences and Technology Project Paper (Bachelor of Applied Science (Honor) in Industrial Chemistry) -- Universiti Malaysia Pahang – 2012 Includes bibliographical references “Patawali” (Malay) refers to Tinospora crispa (Menispermaceae) which has been claimed to have many traditional uses. The root, stem and leaves of this plant have been used for many medicinal purposes such as fever, diabetes and rheumatism. Tinospora crispa contains variety of phytochemicals. In this study, the powdered Tinospora crispa stems were extracted using different solvents and pH to obtain five fractions (Fraction 1, 2, 3, 4 and 5) with varying polarity. These fractions were then screened for cytoxicity test using brine shrimp lethality assay. For each fraction, 3 concentrations were prepared (10, 100 and 1000 μg/mL) and the assay was conducted in triplicates. The result obtained showed only Fraction 3 and 4 were toxic with LC50 value of 41.59 μg/mL and 118.58 μg/mL respectively whereas Fraction 1, 2 and 5 did not exhibited any LC50 value for the range of concentrations prepared. Brine shrimp lethality assay results from this study may be used as preliminary indication as anticancer agent of the likely presence of toxic compounds in those particular fractions. Other cytotoxicity tests and specific bioassays may be done on the isolated bioactive compounds later. Faculty of Industrial Sciences and Technology Dissertations Universities and colleges Dissertations Theses lcc THESIS