03751ntm a2200325 i 4500003000800000005001700008006001900025008004100044020003200085040002300117100003700140245012100177264003400298264001200332300007200344336002100416336002100437337002500458337002300483338002300506338003000529347002400559500005000583502007100633504004000704520256000744610006503304650004503369650001103414MY-KuUP20251125105953.0t||||fr|||| 000 0 220329s2021    my a|||frm||| 000 0 eng d  aTHE0009194(Local)qhardback  aUMPbengcUMPerda1 aYasin M. Y. Albarqouni,eauthor.10aSingle-stranded dna as a dual functioning biomolecule in graphene composite supercapacitor /cYasin M. Y. Albarqouni 1aKuantan, Pahang :bUMP,c2021 4c© 2021  axviii, 97 pages :billustrations (some color) ;c30 cm. +e1 CD-ROM  atext2rdacontent  atext2rdacontent  aunmediated2rdamedia  acomputer2rdamedia  avolume2rdacarrier  acomputer disc2rdacarrier  atext filebPDF2rda  aFaculty of Industrial Sciences and Technology  aThesis (Master of Science ) -- Universiti Malaysia Pahang – 2021  aIncludes bibliographical references3 aThe  enrichment  of  DNA’s  chemistry  endows  DNA  with  a  high  level  of  interest  in  bioelectronics  applications.  This research investigates  the charge storage capability and  mechanism for the synthesis of GO/DNA bio-composite aiming to develop a new type of  supercapacitors  based  on  biomaterial.  To  this  end,  a  sequential  success  of  material  synthesis  confirmed,  starting  with  successful  isolation  of  DNA  from  saccharomyces  cerevisiae  from  broth  culture  extended to  successful oxidation of graphite flakes  using  the  modified  Hummer’s  method  to  further  develop  a  one-step  reduction  and functionalisation  of  graphene  oxide  (GO)  with  different  DNA  forms.  The  composites  physiochemical properties investigated with spectroscopic techniques including UV-Vis, Fourier transform infrared (FTIR),  X-ray diffraction (XRD),  Raman  shift,  and  fluorescent  spectroscopies  (FL).  In  addition,  surface  morphology  after  the  reduction  observed  by  employing FESEM.  Cyclic voltammetry  (CV), galvanostatic charge-discharge  (GCD),  and  electrochemical  impedance  spectroscopy  (EIS)  techniques  employed  in  threeelectrode configurations to study the electrochemical characteristics of the pure GO and  the fabricated composites  including rGO/ssDNA and GO/dsDNA electrodes in 1 M KOH.  rGO/ssDNA working  electrode.  All  of  the mentioned techniques verify the reduction of  GO in the presence of ssDNA as well as the functionalization of  both forms of  DNA  including double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA)  on GO. The  easily  oxidized  guanine  nucleic  acid  acts  as  a  proton  pump  that  reduces  oxygen  functionalities.  All  electrochemical  investigations  confirmed  the  electric  double-layer  capacitance  (EDLC)  effect  for  pure  GO  and  synthesized  composites.  It  is  worth  mentioning  that  the  rGO/ssDNA composite elucidated  the highest EDLC effect compared  to  GO  and  GO/dsDNA  composite.  Specific  capacitance  for  rGO/ssDNA  electrode  is  found  to  elucidates  approximately  20-folds  higher  capacitance  compared  to  other  electrodes with  226 F  g-1at a current density  of  0.4 A  g-1in 1 M KOH. Furthermore,  rGO/ssDNA showed  an advancement electrochemical long-term stability of about 92.8%  after 10000 cycles in the aqueous basic electrolyte is also reported. The work comes with  a  successful  use  of DNA as a  reducing  agent  and conductive  biomolecule in  graphene  composite supercapacitor applications .20aFaculty of Industrial Sciences and TechnologyxDissertations 0aUniversities and collegesxDissertations 0aTheses