Effect of ph and ionic strength on permeate flux during separation of lactobacillus plantarum by using hollow fiber crossflow microfiltration / Nor Anira Abdullah

By: Material type: TextTextPublication details: Kuantan, Pahang : UMP, 2010Description: xvi, 92 p. : ill. (some col.) ; 30 cm. + 1 CD-ROMISBN:
  • THE0004414(Local)
Subject(s): Dissertation note: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010 Abstract: Microfiltration is a separation process used for cell harvesting in downstream process. Current research focused on the factor affecting the process of cell separation from fermentation broth to be recycled into the fermenter. The efficiency of solute separation by microfiltration can be influenced by solution pH and ionic strength. The objectives of this research is to study the effects of pH solution which is pH 4.5, 5.5, 6.5, 7.5, 8.5 and ionic strength on permeate flux of separation of Lactobacillus plantarum bacteria. For this research, the 0.2, 0.4, 0.6, 0.8 and 1.0 M of salt concentration are also used. At pH 8.5, permeate flux is the highest due to the electrostatic repulsion between the Lactobacillus plantarum bacteria and the surface of the membrane. The lowest permeate flux is at 1.0 M of ionic strength due to compaction of membrane and results in reduction of effective permeability. As conclusion, flux can be affected by pH solution and addition of salt. Increase in pH solution resulted in increase in permeate flux and addition of salt decreases permeate flux.
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Item type Current library Call number Copy number Status Date due Barcode
Final Year Report Final Year Report UMPLIB GAMBANG TP248.25.M46 A55 2010 rs Bc. (Browse shelf(Opens below)) 1 Not for loan 0000063277
Final Year Report Final Year Report UMPLIB GAMBANG CD 5953 | TP248.25.M46 A55 2010 rs Bc. (Browse shelf(Opens below)) 1 Not for loan 0000063278

Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010

Bibliography : p. 74-76

Microfiltration is a separation process used for cell harvesting in downstream process. Current research focused on the factor affecting the process of cell separation from fermentation broth to be recycled into the fermenter. The efficiency of solute separation by microfiltration can be influenced by solution pH and ionic strength. The objectives of this research is to study the effects of pH solution which is pH 4.5, 5.5, 6.5, 7.5, 8.5 and ionic strength on permeate flux of separation of Lactobacillus plantarum bacteria. For this research, the 0.2, 0.4, 0.6, 0.8 and 1.0 M of salt concentration are also used. At pH 8.5, permeate flux is the highest due to the electrostatic repulsion between the Lactobacillus plantarum bacteria and the surface of the membrane. The lowest permeate flux is at 1.0 M of ionic strength due to compaction of membrane and results in reduction of effective permeability. As conclusion, flux can be affected by pH solution and addition of salt. Increase in pH solution resulted in increase in permeate flux and addition of salt decreases permeate flux.

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