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008			260121t20252025my a|||fs|||| 000 0 eng d
020			_aTHE0010295 (Local) _qHardback
040			_aUMPSA _beng _cUMPSA _erda
090			_aFIST .Y86 2025 r Bc.
100	1		_aYun Ning, Grace Ng, _eauthor.
245	1	0	_aEffect of murashige and skoog medium strength and plant growth regulators on the growth of momordica cochinchinesis (gac fruit) / _cGrace Ng Yun Ning
264		1	_aKuantan, Pahang : _bUMPSA, _c2025
264		4	_c© 2025
300			_axiv, 86 pages : _billustrations ;
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
347			_2rda _atext file _bPDF
500			_aFaculty of Industrial Sciences and Technology
502			_aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504			_aInclude bibliographical reference
520	3		_aMomordica cochinchinensis, or Gac fruit, is a tropical superfruit known for its high nutritional and medicinal value but faces cultivation challenges such as low seed viability, poor germination, and environmental stress. Tissue culture offers a solution by enabling controlled propagation of elite plants. This study aimed to improve sterilization and optimize growth media for better in vitro propagation. Sodium hypochlorite concentrations for surface sterilization, Murashige and Skoog medium strengths, and cytokinin concentrations were evaluated for their role in affecting shoot growth and proliferation. Best surface sterilization was under 15% NaOCl for 10 minutes where 0% contamination rate was achieved, ensuring aseptic explant preparation. Among the tested MS medium strengths, full, half, and quarter, full-strength MS provided the best results, with the highest number of shoots (6.33 ± 0.33), shoot length (1.80 ± 0.06 cm), and leaf formation (4.67 ± 0.67) after five weeks. The study also tested various BAP concentrations (0, 1.0, 2.0, 3.0, and 4.0 mg/L), with 4.0 mg/L showing the highest shoot proliferation (16.67 ± 0.88), shoot length (4.50 ± 0.17 cm), and leaf number (12.00 ± 1.15), emphasizing the importance of hormonal optimization. The results highlight the significance of optimizing sterilization methods and media formulations to improve Gac fruit tissue culture efficiency, providing a foundation for sustainable cultivation and commercial production. Statistical analysis using One-Way ANOVA (p ≤ 0.05) showed that MS strength significantly affected all growth parameters, while BAP concentration significantly influenced shoot length but had no significant effect on shoot and leaf numbers
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aFinal Year Report _xDissertations
856			_uhttp://umpir.ump.edu.my/id/eprint/45601
942			_2lcc _cPSM
999			_c103902 _d103908