000			02869nam a22003617a 4500
003			MY-KuUP
005			20260126144015.0
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007			ta
008			260126t20252025my a|||fs|||| 000 0 eng d
020			_aTHE0010315 (Local) _qHardback
040			_aUMPSA _beng _cUMPSA _erda
090			_aFIST .H57 2025 r Bc.
100	1		_aNur Shahira Binti Razali, _eauthor.
245	1	0	_aMolecular identification of a fungi isolate from liquid culture of colon cancer biopsy / _cNur Shahira Binti Razali
264		1	_aKuantan, Pahang : _bUMPSA, _c2025
264		4	_c© 2025
300			_axiii, 64 pages : _billustrations ;
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
347			_2rda _atext file _bPDF
500			_aFaculty of Industrial Sciences and Technology
502			_aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504			_aInclude bibliographical reference
520	3		_aFungal infections are increasingly recognized as significant factors affecting various health issues in humans, including cancer. This research aimed to explore fungal species obtained from liquid cultures derived from colon cancer biopsy samples, particularly seeking to identify possible connections between fungal presence and colon cancer pathology. Liquid cultures from biopsies were carefully prepared, and fungal isolates were grown under regulated laboratory conditions. Morphological traits and growth behaviors were documented to ensure precise differentiation of the isolates. Molecular classification of the fungi was conducted utilizing Polymerase Chain Reaction (PCR)- based methods that target specific genetic markers of fungi. DNA sequencing and phylogenetic analysis additionally validated the taxonomic identification of the isolates. The findings indicated the presence of Gymnopilus sp. within the biopsy cultures, with some isolates exhibiting distinct genetic variations. Initial assessments imply possible interactions between the identified fungi and the tumor microenvironment, warranting further study. This research emphasizes the significance of investigating fungal microbiota in cancer studies and highlights the critical need for molecular identification techniques to uncover potential microbial contributors to cancer development. Future research will aim to clarify the mechanistic roles of identified fungal species in the progression of colon cancer, providing insights into innovative diagnostic and therapeutic strategies.
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aFinal Year Report _xDissertations
856			_u _z
942			_2lcc _cPSM
999			_c103927 _d103933