000			02925ntm a2200337 i 4500
003			MY-KuUP
005			20260227101351.0
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008			260227t20252025my a|||fs|||| 000 0 eng d
020			_aTHE0010397 (Local) _qHardback
040			_aUMPSA _beng _cUMPSA _erda
090			_aFIST .S83 2025 r Bc.
100	1		_aSudarmathi A/p Rathakrishnan, _eauthor.
245	1	0	_aDifferential expressed gene associated with growth and aromas analysis in different banana varieties / _cSudarmathi A/p Rathakrishnan
264			_aKuantan, Pahang : _bUMPSA, _c2025
264			_c© 2025
300			_axiii, 73 pages : _billustrations ;
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
347			_2rda _atext file _bPDF
500			_aFaculty of Industrial Sciences and Technology
502			_aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504			_aInclude bibliographical reference
520	3		_aBananas (Musa spp.) are a key agricultural crop, offering essential nutrients and significant economic value globally. In Malaysia, different types of bananas are widely cultivated varieties with distinct growth and aromatic characteristics. This study analyzed differential gene expression related to growth and aroma in three Malaysian banana varieties, which are Pisang Mas (Musa acuminata), Pisang Tanduk (Musa paradisiaca), and Pisang Rastali (Musa sapientum). Key target genes investigated were Xyloglucan Endotransglucosylase/Hydrolase (XTH), associated with fruit softening and growth, and Alcohol Dehydrogenase (ADH), critical in aroma compound biosynthesis. Total RNA was extracted from the mesocarp of ripe and unripe samples using the RNA extraction kit, followed by complementary DNA (cDNA) synthesis. Primer sequences for XTH and ADH were designed using data from the GenBank database. Gel electrophoresis confirmed the integrity of the extracted RNA, and UV spectrophotometry verified its concentration and purity. PCR analysis revealed significant variations in gene expression among the banana varieties and across ripening stages. PCR amplification showed that Pisang Mas exhibited robust expression of both XTH and ADH genes across ripening stages. In Pisang Tanduk, XTH expression was prominent, while ADH expression was lower. For Pisang Rastali, both XTH and ADH expressions were comparatively lower than other bananas. This study highlights the potential of leveraging gene expression data for crop improvement, such as enhancing fruit quality, extending shelf life, and improving aroma profiles.
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aFinal Year Report _xDissertations
942			_2lcc _cPSM
999			_c104248 _d104254