000			02860nam a2200337 i 4500
003			MY-KuUP
005			20260227150219.0
006			t||||fr|||| 000 0
007			ta
008			260227t20252025my a|||fr|||| 000 0 eng d
020			_aTHE0010402 (Local) _qHardback
040			_aUMPSA _beng _cUMPSA _erda
090			_aFIST .A34 2025 r Bc.
100	1		_aAieda Farihin Binti Bakhary, _eauthor.
245	1	0	_aEvaluating the antifungal effects of crude protease extracted from lactic acid bacteria / _cAieda Farihin Binti Bakhary
264		1	_aKuantan, Pahang : _bUMPSA, _c2025
264		4	_c© 2025
300			_axii, 95 pages : _billustrations ;
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
347			_2rda _atext file _bPDF
500			_aFaculty of Industrial Sciences and Technology
502			_aFinal Year Report (Bachelor of Applied Science in Industrial Biotechnology) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504			_aInclude bibliographical reference
520	3		_aCandida species, particularly Candida albicans, are the primary causes of invasive candidiasis. The scarcity of therapeutic choices and the growing frequency of antifungal resistance highlight the critical need for new treatments. This study aimed to investigate the antifungal activity of lactic acid bacteria (LAB) isolates obtained from pickled maman, Cleome gynandra. A total of 10 LAB isolates were screened using a dual agar overlay assay to evaluate their antifungal activity based on zones of inhibition against C. albicans. The C. albicans used was confirmed via Germ Tube Test. All 10 isolates showed antifungal activity against C. albicans, ranging from weak to strong inhibition. Three isolates (M5, M6, M9) produced the largest zones of inhibition with 17 ± 1.73 mm, 14 ± 0.57 and 18 ± 0.57 mm, respectively. These LAB isolates were further analyzed for protease enzyme activity using the Lowry method on their cell-free supernatants. All three LAB isolates were shown to produce protease activity. M6 had the highest activity (1.212 × 10-5 U/mL), followed by M5 (8.4 × 10-6 U/mL) and M9 (4.7 × 10-6 U/mL). These results demonstrated that specific LAB isolates possess significant antifungal activity and their abilities to produce protease enzymes. These findings are preliminary report suggesting LAB isolates from pickled maman shows promising potential as natural therapeutic agents against resistant strains of Candida. Further research is warranted to unravel their antifungal mechanisms and assess their viability in clinical and industrial settings
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aFinal Year Report _xDissertations
942			_2lcc _cTHESIS
999			_c104253 _d104259