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020 _aTHE0010618 (Local)
_qHardback
040 _aUMPSA
_beng
_cUMPSA
_erda
090 _aFTKKP .M87 2024 r Bc.
100 0 _aMus’ifah Nabilah Binti Mohd Nizam ,
_eauthor.
245 1 0 _aMedia formulation and its characterization for inoculum development of wild strain acetic ACID bacteria (AAB) /
_bMus’ifah Nabilah Binti Mohd Nizam
264 1 _aKuantan, Pahang :
_bUMPSA,
_c2024
264 4 _c© 2024
300 _axiv, 51 pages :
_billustrations ;
336 _2rdacontent
_atext
337 _2rdamedia
_aunmediated
338 _2rdacarrier
_avolume
347 _2rda
_atext file
_bPDF
500 _aFaculty of Chemical and Process Engineering Technology
502 _aFinal Year Report (Bachelor of Chemical Engineering ) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2024
504 _aInclude bibliographical reference
520 3 _aAcetic acid has been produced through fermentation through the oxidation of ethanol using Acetobacter spp., known as acetic acid bacteria (AAB). The current challenge in this research lies in identifying the specific requirements for inoculum development of wild strain AAB because to increase the acetic acid production, growth factors need to be optimized using statistical analysis. So, this study will be focuses on varying glucose concentrations to understand the correlation between carbon availability and AAB growth, followed by the characterization of inoculum. To achieve this objective, a wild strain of AAB isolated from MD2 pineapple waste will be used to explore the growth characteristics and suitability for inoculum development. The investigation will focus on the impact of varying glucose concentrations (30, 50, 60) in g/L as the carbon source on the growth of AAB cells and further undergo a detailed characterization, including key parameters such as motility, Gram staining, and the potency index (PI). The results indicate that the optimal sugar concentration for the experiment is at 50 g/L, where the OD is 0.4065, glucose consumption is 27.5, and the PI value is 1.83. The inoculum, grown in the media, produced a Gram-negative coccobacillus (rod shape) with no specific arrangement under these favourable conditions. These characterization methods arc hosed for their relevance evaluating the quality of the inoculum. Keywords: Acetic Acid Bacteria; Inoculum development; Carbon availability; growth; characterization
610 2 0 _aFaculty of Chemical and Process Engineering Technology
_xDissertations
650 0 _aUniversities and colleges
_xDissertations
650 0 _aTheses
_xDissertations
856 _uhttps://umpir.ump.edu.my/id/eprint/47026
942 _2lcc
_cPSM
999 _c105016
_d105022