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008			260710t20252025my a|||fr|||| 000 0 eng d
020			_aTHE0010703 (Local) _qHardback
040			_aUMPSA _beng _cUMPSA _erda
090			_aFTKKP .I57 2025 r Bc.
100	0		_aNurin Rasyiqah Binti Mohd Rashdan, , , _eauthor.
245	1	0	_aStudy on the enzyme kinetics of xylanase in poultry feed hydrolysis / _cNurin Rasyiqah Binti Mohd Rashdan
264		1	_aKuantan, Pahang : _bUMPSA, _c2025
264		4	_c© 2025
300			_axv, 46 pages : _billustrations ;
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
347			_2rda _atext file _bPDF
500			_aFaculty of Chemical and Process Engineering Technology
502			_aFinal Year Report (Bachelor of Chemical Engineering ) -- Universiti Malaysia Pahang Al-Sultan Abdullah - 2025
504			_aInclude bibliographical reference
520	3		_aPoultry is a common source of protein that is essential for our daily sustenance. Worldwide poultry production has expanded in response to rising demand for poultry meat and eggs due to population, urbanization and industrialization. Poultry lacks the digestive enzymes necessary to fully digest fiber. Hence, they require commercial exogenous enzymes in their diets to aid digestion. Adding enzymes to poultry feed enhances enzyme-substrate binding efficiency, thereby improving the formulation of commercial poultry feed. The main objective of this research is to investigate the enzyme kinetics of commercial xylanase in poultry feed hydrolysis. The experiments were conducted with varying substrate concentrations (2%, 4%, 6%, 8%, and 10% (w/v)) and pH (4, 5, 6, 7 and 8) at a 120 minutes of reaction time. It is done by reacting poultry feed with xylanase enzyme. The enzymatic reaction was then stopped by adding dinitrosalicylic acid (DNS) reagent. The absorbance was read using a UV-Vis spectrophotometer. Further analysis for optimization of enzyme activity using Central Composite Design (CCD) and the kinetic parameters (Km and Vmax) were analyzed using the Michaelis-Menten method. The best results were found at 8% (w/v) substrate concentration and the pH 6 which the highest xylanase activity was at 1.0829 U/ml (xylan corn cob) and 1.9370 U/ml (poultry feed). At pH 6, xylan corn cob shows a higher Vmax (1.0536 mol min-1 mg-1) and lower Km (0.8150 mg/ml) compared to poultry feed (Vmax = 0.4294 μmol min-1 mg-1, Km = 9.0081 mg/ml), indicating greater catalytic efficiency and substrate affinity. In conclusion, understanding enzyme reaction rate and substrate interactions can help increase the degradation of complex non-starch polysaccharides (NSPs) and improve nutrient release and utilization in poultry. This, in turn, can help minimize the integration of costly feed supplements into poultry feed formulation, thus providing a cost-effective and sustainable poultry feed formulation.
610	2	0	_aFaculty of Chemical and Process Engineering Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aTheses _xDissertations
856			_uhttps://umpir.ump.edu.my/id/eprint/47568
942			_2lcc _cPSM
999			_c105170 _d105176