000			05059ntm a2200337 i 4500
003			MY-KuUP
005			20261007092717.0
006			t||||fr|||| 000 0
007			ta
008			261005t20262026my a|||fr|||| 000 0 eng d
020			_aTHE0010811 (Local) _qHardback
040			_aUMPSA _beng _cUMPSA _erda
090			_aFSTI .F38 2026 r Thesis
100	1		_aNur Fatini Ilyana Mohamat Johari, _eauthor.
245	1	0	_aSilver nanoparticles-edible bird nest embedded nanofibre for wound healing agent / _cNur Fatini Ilyana Mohamat Johari
264		1	_aKuantan, Pahang : _bUMPSA, _c2026
264		4	_c© 2026
300			_axvi, 168 pages : _billustrations ; _c30 cm. + _e1 CD-ROM.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
347			_2rda _atext file _bPDF
500			_aFaculty of Industrial Sciences and Technology
502			_aThesis (Master of Science) -- Universiti Malaysia Pahang - 2026
504			_aInclude bibliographical reference
520	3		_aResearch on biomaterials in the form of nanofibers for tissue engineering has seen significant growth, primarily because these materials closely mimic the extracellular matrix (ECM), which supports cell attachment, differentiation, and proliferation. This study focuses on edible bird’s nest (EBN), a natural substance known for its longstanding medicinal properties, as the core material for nanofiber development. EBN contains bioactive compounds that enhance nanoparticle formation and promote cell differentiation. The objective of this research is to develop nanofibers composed of EBN integrated with silver nanoparticles (AgNPs) for applications in skin tissue engineering. Electrospinning, a technique capable of producing nanometer-scale fibers with interconnected pores resembling ECM structure, was employed for fiber fabrication. This report is divided into two main sections. The first part covers the fabrication and characterization of the nanofibers, while the second focuses on cell culture and animal studies using both the nanofibers and EBN solution. In the first phase, five different EBN concentrations (3–7%) were used to synthesize AgNPs. The Ag-EBN solution was characterized using Ultraviolet visible (UV-Vis) spectrophotometry, Quadrupole Time-of-Flight Liquid Chromatograph Mass Spectrophotometer (LC-QTOF MS), and Field Emission Electron Microscope (FESEM). An absorption peak at 460 nm indicated the presence of AgNPs via surface plasmon resonance. LC-QTOF MS identified Citrusin C, a phenolic compound in EBN, as the agent responsible for reducing and stabilizing AgNPs. The nanoparticles measured between 17 and 35 nm in diameter. The optimized Ag-EBN solution was mixed with 10 wt% polyvinyl alcohol (PVA) in a 20:80 ratio and electrospun to produce smooth nanofibers. To render the nanofibers water-insoluble, they were chemically crosslinked using glutaraldehyde. The nanofibers’ microstructure, morphology, mechanical, and thermal properties were analyzed using FESEM, Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-ray Diffraction (XRD), ultimate tensile strength (UTM), and Thermogravimetric analysis (TGA). ATR-FTIR results indicated possible interactions between Ag-EBN and PVA. FESEM revealed fiber diameters ranging from 75 to 100 nm. XRD analysis showed a peak shift from 19.70° for pure PVA and observed shift peak with Ag-EBN, indicating structural changes. TGA/DTGA curves displayed distinct weight-loss stages across different fiber concentrations. The highest Young’s modulus was observed at 6% Ag-EBN, indicating improved in fibre stiffness. The biological properties, including antibacterial activity and in vitro wound healing, were also assessed. Notably, 7% Ag-EBN/PVA nanofibers exhibited the strongest antibacterial effect against Staphylococcus aureus. However, when tested in a 96-well plate for in vitro wound closure, AgNP-containing nanofibers were found to be cytotoxic to myocytes, showing no wound healing activity. The second part of the study explored in vitro and in vivo evaluations of EBN solution, EBN/PVA nanofibers, and PVA nanofibers. Wound healing assay were assessed using live imaging microscopy. Among the tested formulations, 5% EBN/PVA nanofibers achieved the highest wound closure after 24 hours, indicating excellent cell compatibility due to PVA incorporation. For the in vivo study, diabetic rats were treated with the different formulations to monitor wound reduction. After 14 days, the group treated with 5% EBN/PVA nanofibers showed complete wound healing compared to the other groups. Histological analysis confirmed re-epithelialization across all treated wounds. These findings suggest that the biocompatible EBN-based nanofibers hold great potential for use in next-generation tissue-engineered wound dressings.
610	2	0	_aFaculty of Industrial Sciences and Technology _xDissertations
650		0	_aUniversities and colleges _xDissertations
650		0	_aTheses _xDissertations
942			_2lcc _cTHESIS
999			_c105669 _d105675