000			02857nam a2200253 a 4500
001			vtls000031354
003			KUKTEM
005			20251114204413.0
008			080902t2008 my a f m 000 0 eng|d
020			_aTHE0002257(Local)
039		9	_a201905241126 _bshamsul _c201302011510 _dhuda _c201107132227 _dVLOAD _c200908141515 _dVLOAD _y200809021445 _zida84
040			_aUMP
090			_aQP609.L5 H37 2008 rs Thesis
100	0		_aHasrul Azmi Said
245	1	0	_aImmobilization of lipase from Candida Rugosa on chitosan beads for transesterification reaction / _cHasrul Azmi Said
246	3		_aImmobilization of lipase from Candida Rugosa on chitosan beads for transesterification reaction _h[electronic resource]
260			_aKuantan, Pahang : _bUMP, _c2008
300			_a53 p. : _bill. (some col.) ; _c30 cm. + _e1 computer disc
502			_aProject paper (Bachelor of Chemical Engineeering) -- Universiti Malaysia Pahang - 2008
520	3		_aThe objective of this study was to evaluate the immobilization of lipase on a chitosan support by physical adsorption, aiming its application in transesterification reactions. Immobilization of lipase give several advantages such as improved stability, reuse, continuous operation and the possibility of better control of reactions. Chitosan offers several advantages as an enzyme immobilization carrier such as versatility of available physical forms (flakes, porous beads, gel, fiber and membrane), low biodegradability and easy handling. In this study, bead porous of chitosan was used for immobilizing lipase from a microbial source of Candida rugosa. Lipase was immobilized by physical adsorption on chitosan following a previously developed methodology by Carneiro da Chunca et al. (1999). The ability of immobilized lipase on chitosan beads to catalyze transesterification of cooking oil and methanol was investigated. The important parameters like reaction time and oil to methanol molar ratios were studied to indentify the comparison between free lipase and immobilized lipase on transesterification reaction. From the study it was found that maximum conversion of ester using immobilized lipase and free lipase were 72.25% and 76.5% obtained at the optimum conditions of 1:4 molar ratios and reaction time of 48 hour. Therefore, the conversion of ester for free lipase was higher than immobilized lipase. As a conclusion, the chitosan beads were appear as a suitable support for immobilized lipase on trasnesterification reaction even though the ester conversion was lower than free lipase. On the other hand, immobilized lipase was provided an important advantage such as easy separation from the product and has a high potential to reuse.-Author
650		0	_aLipase
650		0	_aChitosan
999			_aVIRTUA40 _c1445 _d1451
999			_aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*6501*9992