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003 KUKTEM
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008 070308t2006 my a f m 000 0 eng d
020 _aTHE0002260(Local)
039 9 _a201905241129
_bshamsul
_c201211021701
_dFida
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_dVLOAD
_c200908141342
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_zfarina
040 _aUMP
090 _aQP609.P78 M64 2006 rs Thesis
100 0 _aMohd Fadli Abdullah
245 1 4 _aThe production of extracellular protease using bacillus subtilis :
_beffect of temperature and agitation speed /
_cMohd Fadli Bin Abdullah
260 _aKuantan , Pahang :
_bKUKTEM ,
_c2006
300 _ax, 56 p. :
_bill. ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering) -- Kolej Universiti Kejuruteraan dan Teknologi Malaysia - 2006
504 _aBibliography: p. 43-45
520 _aProduction of extracellular protease refers to proteases produced from microorganisms and functions out of the cell in which it was originated. In this term, the microorganism was sought to be normal mesophilic bacteria. The experiment was conducted to evaluate the effects of temperature and agitation speed of the incubator shaker towards the production of extracellular protease. Optimization of the enzyme production condition involving the two parameters was also made using statistical software. Typical methods conducted in previous researches were utilized in the experiments using different temperature and agitation speed. However, in previous researches, experiments were conducted through one-factor-at-a-time method (OF AT) but in this experiment, that method was overrun and designed beforehand using Minitab. The standard procedures of protease assay were carried out when experimenting. The bacteria were re-cultured to enable its functionality to serve its purpose. The results obtained proved that both parameters had significant effects if stood alone towards the production, however had to interact with each other due to randomization of the values implemented in the experiments. Simple analysis was not an option therefore the results were opted to be analyzed by Minitab and also the condition of protease production was optimized.
650 0 _aProteolytic enzymes
650 0 _aTemperature control
999 _aVIRTUA40
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