000 01786nam a2200241 a 4500
001 vtls000057155
003 KUKTEM
005 20251114204417.0
008 111212t2010 my dao f m 000 0 eng d
020 _aTHE0004145(Local)
039 9 _a201905132129
_bfarhana
_y201112121539
_zFida
040 _aUMP
090 _aTP156.F5 Z53 2010 rs Bc.
100 0 _aZianor Azrina Zianon Abdin
245 1 0 _aWhey protein fractionation based on q-sepharose anion exchange chromatography /
_cZianor Azrina Zianon Abdin
260 _aKuantan, Pahang :
_bUMP,
_c2010
300 _axi, 50 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering) -- Universiti Malaysia Pahang - 2010
504 _aBibliography: p. [40]-41
520 _aWhey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, βlactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose anion exchange chromatography column. The chromatographic process was run at different pH range from pH5 to pH8. The best separation was achieved at pH6 in which the major protein component can be separated into their individual protein fraction especially contains αlactalbumin and β-lactoglobulin. The yield of α-lactalbumin and β-lactoglobulin recovered from 2 ml whey at pH 6 was 46% and 98.4% respectively. Fractionation of single and pure protein component from whey can add the value of the wheybased product and these proteins can be used for specific application.
650 0 _aUltrafiltration
999 _aVIRTUA40
_c1569
_d1575
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2600*3000*5020*5040*5200*6500*9992