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003 KUKTEM
005 20251114204420.0
008 100128t2009 my a f m 000 0 eng|d
020 _aTHE0004110(Local)
039 9 _a201905131405
_bfarhana
_c201107132306
_dVLOAD
_c201002091040
_dida
_c201001281233
_dida
_y201001281108
_zida
040 _aUMP
090 _aTP156.F4 A36 2009 rs Bc.
100 0 _aAimi Salma Awalludin
245 1 0 _aScale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2L stirred tank fermentor /
_cAimi Salma Awalludin
246 3 _aScale up of biopolymer (PHB) fermentation from 500Ml shake flasks to 2Lstirred tank fermentor
_h[electronic resource]
260 _aKuantan, Pahang :
_bUMP,
_c2009
300 _a51 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of of Chemical engineering) -- Universiti Malaysia Pahang - 2009
520 3 _aThis study is about the scale up of biopolymer fermentation from 500 mL shake flasks to 2L fermentor. The purpose of this project is to determine air flow rate at 2L fermentor which gives the same kLa and kap as produced in shake flasks. The rationale of using the kLa value is to ensure a certain mass transfer capability in order to cope with the oxygen demand of the culture, thus being an important scale-up factor. Both values were derived by fitting the dissolved oxygen tension (DOT) versus time data into The Fibonacci Min Search (Fminsearch) method. Two values, which are 0.2960 for volumetric mass transfer coefficient for oxygen (kLa) and 0.0220 for the electrode mass transfer coefficient (kap) were obtained at small scale. These values are required to be duplicated in the larger scale. It was done by using a fixed agitation rate of 200 rpm and a manipulated aeration rate which is 1L/min,1.5L/min,2L/min and 1.75L/min. The most comparable kLa and kap values obtained from the trial are at 1.75L/min. This aeration rate will be used in the 2L fermentor in order to investigate the production of poly-β-hydroxybutyrate (PHB). Fermentation is run at both scales to compare the glucose, biomass and PHB profile. From the experiment and calculation, the maximum concentration of PHB is achieve at the 36th hours, which is 1.415g/L for 500 mL shake flasks, and 2.17g/L for 2L fermentor. The cell dry mass obtained at the optimum harvesting time is 6.065 g/L for both scales.-Author-
650 0 _aFermentation
650 0 _aBiopolymers
999 _aVIRTUA40
_c1665
_d1671
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*6501*9992