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008 100205t2009 my a f m 000 0 eng d
020 _aTHE0004529(Local)
039 9 _a201905131540
_bazhar
_c201108030911
_dFida
_c201107132306
_dVLOAD
_c201002081445
_dkam
_y201002051520
_zkam
040 _aUMP
090 _aTP248.65.P76 J36 2009 rs Bc.
100 0 _aJamilah Junuh
245 1 2 _aA comparative study on protein separation using different techniques /
_cJamilah Binti Junuh
246 3 _aComparative study on protein separation using different techniques
_h[computer file]
260 _aKuantan :
_bUMP,
_c2009
300 _a47 p. :
_bill. (some col.) ;
_c30 cm. +
_e1 computer disc
502 _aProject paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2009
520 3 _aConcentration of commercial soy milk into protein stream using crossflow filtration unit with ultrafiltration membrane and conventional method was examined. Two membranes of molecular weight cut off (MWCO) of 10 and 50 kilo Dalton (kDa) of regenerated polysulfone material were used to determine the better techniques and efficiency of the process. The performance was determined under various processing conditions that include the operating transmembrane pressure the pore size of the membrane. Partially soymilk was centrifugated at 8000 rpm and 30 minutes in 20oC to remove the colloidal materials to prevent the membrane fouling. It is found that the permeate flux for 10kDa membrane are decreased with time. This is due to fouling of the membrane. An increased in transmembrane pressure increased the permeate flux and protein weight. The 10kDa membrane shows that the ability to adsorb more amount protein instead of 50kDa membrane. The weight of protein being low at lower transmembrane pressure (less than 10psig) and high at higher transmembrane pressures (greater than 15 psig). The amount of protein recovered at greater transmembrane pressure is more than 5 grams. The method of isolation (conventional method) were adjusted the pH of soy milk (dilute with distilled water) to 4.6 at 20oC. Used this method gives a weight of protein in not more than 5 grams. It is found that the best membrane to retain highest weight of protein is 10kDa.
650 0 _aProteins
_xSeparation
650 0 _aSeparation (Technology)
999 _aVIRTUA40
_c1666
_d1672
999 _aVTLSSORT0080*0200*0400*0900*1000*2450*2460*2600*3000*5020*5200*6500*6501*9992